B7-H3 CAR T-cell therapy for pediatric solid tumors

B7-H3-Specific Chimeric Antigen Receptor Autologous T-Cell Therapy for Pediatric Patients With Solid Tumors (3CAR)

Phase 1 Interventional St. Jude Children's Research Hospital · NCT04897321

This study is testing a new type of immune therapy using specially modified T cells to see if it can help children with tough-to-treat solid tumors.

Quick facts

PhasePhase 1
Study typeInterventional
Enrollment48 (estimated)
AgesN/A to 21 Years
SexAll
SponsorSt. Jude Children's Research Hospital Academic / other
Drugs / interventionsCAR T, chemotherapy, immunotherapy
Locations1 site (Memphis, Tennessee)
Trial IDNCT04897321 on ClinicalTrials.gov

What this trial studies

This clinical trial investigates the use of genetically engineered T cells that target the B7-H3 protein in pediatric patients with relapsed or refractory solid tumors. It is a Phase I trial that aims to determine the safety and maximum tolerated dose of these B7-H3-CAR T cells following lymphodepleting chemotherapy. The study will also evaluate the antitumor activity of the treatment and assess changes in the tumor environment post-infusion. Participants will receive a single infusion of the CAR T cells, and the study will last for one year, including a follow-up period.

Who should consider this trial

Good fit: Ideal candidates are pediatric patients aged 21 years or younger with measurable B7-H3-positive solid tumors.

Not a fit: Patients with primary immunodeficiencies or severe infections may not benefit from this study.

Why it matters

Potential benefit: If successful, this therapy could provide a new treatment option for children with difficult-to-treat solid tumors.

How similar studies have performed: Other studies using CAR T-cell therapy have shown promising results, indicating potential success for this approach in treating solid tumors.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

Procurement and T-cell production eligibility\*

\*a previously collected, autologous leukapheresis product can be used for T-cell production

* Age ≤21 years old
* B7-H3+ solid tumor with measurable disease; B7-H3 expression will be evaluated by standard immunohistochemistry (IHC) using a previously obtained biopsy; a tumor is considered B7-H3 positive with an H-score ≥100
* Estimated life expectancy of \>12 weeks
* Karnofsky or Lansky (age-dependent) performance score ≥50
* For females of child bearing age:
* Not pregnant with negative serum pregnancy test within 7 days prior to enrollment
* Not lactating with intent to breastfeed
* Meets eligibility criteria to undergo autologous apheresis, or have previously undergone autologous apheresis

Exclusion Criteria:

* Known primary immunodeficiency
* Known HIV positivity
* Severe intercurrent bacterial, viral or fungal infection (e.g. active hepatitis B or C infection or adenovirus infection)
* History of hypersensitivity reactions to murine protein-containing products
* Rapidly progressive disease (in the opinion of the study PIs)

Inclusion criteria

Treatment eligibility

* Age ≤21 years old
* B7-H3+ solid tumor with measurable disease
* Evidence of relapsed or refractory disease after standard first-line therapy
* Estimated life expectancy of \>8 weeks
* Karnofsky or Lansky (age-dependent) performance score≥50
* Echocardiogram with a ventricular ejection fraction
* \>40%; or shortening fraction ≥25%
* Adequate renal function defined as creatinine clearance or radioisotope GFR 50 ml/min/1.73m2 (GFR 40 ml/min/1.73m2 if \< 2 years of age)
* Adequate pulmonary function defined as pulse oximetry ≥92% on room air or forced vital capacity (FVC) ≥50% of predicted value
* Total Bilirubin ≤3 times the upper limit of normal for age, except in subjects with Gilbert's syndrome
* Alanine aminotransferase (ALT) or aspartate aminotransferase (AST) ≤5 times the upper limit of normal for age
* Hemoglobin≥ 7g/dL (can be transfused)
* Platelet count \>50,000/uL (can be transfused)
* Absolute neutrophil count (ANC) ≥ 1000/uL
* Has recovered from all NCI CTAE grade III-IV, non-hematologic acute toxicities from prior therapy
* For females of child bearing age:
* Not pregnant with negative serum pregnancy test within 7 days prior to enrollment
* Not lactating with intent to breastfeed
* If sexually active, agreement to use birth control until 3 months after T-cell infusion. Male partners should use a condom.
* Available autologous transduced T-cell product that has met GMP release criteria
* Agreement to participate in long-term follow-up protocol for patients, who have received genetically modified cell products

Exclusion criteria

* Known primary immunodeficiency
* History of HIV infection
* Severe, uncontrolled intercurrent bacterial, viral or fungal infection
* History of hypersensitivity reactions to murine protein-containing products
* Receiving systemic steroid therapy exceeding the equivalent of 0.5 mg/kg/day of methylprednisolone, in the 7 days prior to B7-H3-CAR T-cell infusion
* Receiving systemic therapy in the 14 days prior to CAR T-cell infusion, which will interfere with the activity of the B7-H3-CAR product (in the opinion of the study PIs).
* Rapidly progressing disease (in the opinion of the study PIs)

Where this trial is running

Memphis, Tennessee

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Pediatric Solid TumorOsteosarcomaRhabdomyosarcomaNeuroblastomaEwing SarcomaWilms TumorAdrenocortical CancerDesmoplastic Small Round Cell Tumor
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.