Smoking, MFN1, and NLRP3 Levels in Gum Fluid Across Periodontal Health and Disease

Is this trial a fit for you?

A plain-English reading of the study's rules. Only the study team can confirm whether you qualify.

What this trial studies

This observational study compares the levels of MFN1 and NLRP3 in gingival crevicular fluid across four groups: healthy nonsmokers, healthy smokers, nonsmokers with periodontitis, and smokers with periodontitis. Adults 25–65 years old provide medical and smoking histories and undergo a periodontal exam, with gum-fluid samples collected before treatment. The collected samples are analyzed for MFN1 and NLRP3, and results are examined in relation to periodontal measurements and smoking history. The aim is to understand how smoking and periodontal disease together influence these inflammatory and cellular-process proteins.

Why it matters

Who can join: the full rules

Inclusion Criteria:

* Aged 18-65 years. At least 20 natural teeth. For the periodontal health groups: periodontal health on an intact periodontium, with probing depths ≤3 mm, full-mouth bleeding on probing \<10%, and no interdental clinical attachment loss or radiographic bone loss attributable to periodontitis.

For the periodontitis groups: Stage III or Stage IV periodontitis according to the 2017 periodontal disease classification.

For the smoking groups: current cigarette smoking of at least 10 cigarettes per day for at least 5 years.

For the nonsmoking groups: fewer than 100 cigarettes smoked during the lifetime and no current use of tobacco or nicotine products.

Provision of written informed consent.

Exclusion Criteria:

* ormer cigarette smoking. Use of electronic cigarettes, heated tobacco products, nicotine pouches, or nicotine replacement products.

Uncontrolled diabetes, immunodeficiency, autoimmune or chronic inflammatory disease, or a serious systemic disease that may affect periodontal or mitochondrial responses.

Pregnancy or breastfeeding. Use of antibiotics, systemic corticosteroids, immunosuppressive medications, or regular anti-inflammatory medications within the previous 3 months.

Periodontal treatment within the previous 6 months. Acute oral infection, periodontal abscess, or local pathology affecting the sampling sites.

Gingival crevicular fluid samples contaminated with blood or saliva, or insufficient for ELISA analysis.

Where it's running

• Faculty of Dentistry, Bolu Abant İzzet Baysal University, Department of Periodontics
  Bolu, Central, Turkey (Türkiye) Recruiting

Study contacts

• Study coordinator: Zeynep Akgul, DDS, PhD
• Email: zeyneppakgulakgul@gmail.com
• Phone: 05304253826

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How to take part

1. Review the eligibility criteria above with your treating physician.
2. Check the official listing on ClinicalTrials.gov for the latest contact details and recruitment status.
3. Contact the study coordinator or principal investigator to ask for pre-screening. Pre-screening is free and never obligates you to enroll.