Anal cancer screening for men on PrEP

Screening for Anal Cancer in MSM Using PrEP

Not applicable Interventional Universitair Ziekenhuis Brussel · NCT07029152

This study will test how well anal swabs and DNA methylation testing find high-grade anal lesions (HSIL) and how common those lesions are in men who have sex with men using PrEP, and whether HSIL affects quality of life.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment296 (estimated)
Ages35 Years and up
SexMale
SponsorUniversitair Ziekenhuis Brussel Academic / other
Locations8 sites (Bruges and 7 other locations)
Trial IDNCT07029152 on ClinicalTrials.gov

What this trial studies

This multicenter study enrolls HIV-uninfected men who have sex with men aged 35 or older who have used PrEP for at least three months. Participants complete health and quality-of-life questionnaires, provide an anal smear, and undergo high-resolution anoscopy (HRA) with biopsy when indicated. Anal cytology results will be compared to HRA/biopsy findings to measure screening accuracy, and DNA methylation testing will be run on samples to explore molecular markers for HSIL. The study will estimate HSIL prevalence in this PrEP-using population and describe any associated quality-of-life impacts.

Who should consider this trial

Good fit: Ideal candidates are HIV-uninfected men who have sex with men aged 35 or older who have been taking PrEP for at least three months and can speak Dutch, English, or French.

Not a fit: People who are HIV-positive, younger than 35, not using PrEP, or unable/unwilling to attend in-person HRA visits are unlikely to benefit from this study.

Why it matters

Potential benefit: If successful, the study could improve early detection of precancerous anal lesions in PrEP-using men and help tailor screening strategies to reduce future anal cancer risk.

How similar studies have performed: Anal cytology and HRA are established screening approaches for detecting HSIL, while DNA methylation testing is a newer, promising technique with encouraging results in related HPV-driven lesions but is still emerging for anal disease.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* HIV-uninfected MSM (men who have sex with men) aged 35 years or older.
* participants must have been using PrEP for at least 3 months.
* Dutch, English or French speaking and writing

Exclusion Criteria:

* Any intervention in the (peri-)anal region within the past 3 months
* Enema usage within 2 h before sampling
* Currently undergoing peri-anal topical HPV-treatment
* HRA in the last year (anal swab or HRA prior to the last year is no exclusion)

Where this trial is running

Bruges and 7 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Anal CancerSquamous Intraepithelial LesionsHSIL, High Grade Squamous Intraepithelial LesionsLSIL, Low-Grade Squamous Intraepithelial LesionsHPVSquamous Cell CarcinomaHIV preventionmen who have sex with men
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.