Site-specific insertion of large DNA into mouse and human stem cells using Cas9-Bxb1 tools
Official title: Site-specific Integration of Large (10-100 kb) DNA Constructs into the Mouse Genome and Human Induced Pluripotent Stem Cells Using the Cas9-Bxb1 Integrase Toolbox
Funded at Jackson Laboratory · NIH-11370127 · Data updated 2026-11-12
A research project aims to improve how we insert large DNA pieces into mice and human stem cells to better model breast cancer and other diseases.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Jackson Laboratory NIH-funded |
| Lab location | 1 site (Bar Harbor, United States) |
| Project start | July 1, 2022 |
| Project end | June 30, 2027 |
| Funding year | 2026 |
| Award (this year) | $660,883 |
| NIH project number | 5R01CA265978-05 |
| Project ID | NIH-11370127 on NIH RePORTER |
Who could benefit from this research
✓Could help
Not applicable to patient enrollment in this grant; the work focuses on model systems using mouse and human stem cells.
✕Less likely to help
Patients with diseases are not recruited for this basic modeling work.
What this research studies
This project develops a new toolbox that combines CRISPR-Cay9 with the Bxb1 integrase to efficiently insert large DNA sequences into mouse embryos, mouse embryonic stem cells, and human induced pluripotent stem cells. By enabling insertion of big constructs (up to tens of kilobases), it aims to create more faithful models of human cancers and other diseases. The work includes validating the approach in three aims and demonstrating precise integration in relevant cell types that could underpin future cancer research and therapeutic studies.
Why it matters
Potential benefit: If successful, researchers will have a faster, more accurate way to model complex genetic changes tied to cancer, which could accelerate discovery and testing of new treatments.
How similar studies have gone: Preliminary results show the toolbox can integrate up to ~43 kb; this project seeks to broaden capability and validation.
Where this research is happening
-
Jackson Laboratory
Bar Harbor, United States Active
Researchers
- Principal investigator: Hosur, Vishnu — Jackson Laboratory
- Study coordinator: Hosur, Vishnu
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.