Drug or treatment

Methotrexate clinical trials

• 62Clinical trials
• 27Countries

Newest trials

All recruiting trials (62)

• Can older adults with breast cancer tolerate a dense CMF chemo schedule

  This study tests whether giving a condensed CMF chemotherapy regimen is feasible for people 70 and older with breast cancer.

  Recruiting at 7 sites Phase 2 Memorial Sloan Kettering Cancer Center · Basking Ridge, New Jersey and 6 other locations · NCT07768436

• Anti-CD25 antibody to prevent acute GVHD after haploidentical stem cell transplant

  This study tests whether an anti-CD25 antibody can better prevent acute graft-versus-host disease after haploidentical hematopoietic stem cell transplantation compared with the usual methotrexate-based approach.

  Recruiting Phase 3 Union Hospital, Tongji Medical College, Huazhong University of Science and Technology · Wuhan, Hubei · NCT07689630

• R-PMDT Regimen for Newly Diagnosed PCNSL

  This study tests the R-PMDT treatment approach in people newly diagnosed with primary CNS lymphoma to see how many respond.

  Recruiting Phase 2 Institute of Hematology & Blood Diseases Hospital, China · Tianjin · NCT07604987

• Intrathecal methotrexate-loaded tumor-derived vesicles for leptomeningeal lung cancer

  This treatment will try giving methotrexate-loaded tumor-derived vesicles into the spinal fluid to see if it is safe and helps adults with leptomeningeal metastasis from lung cancer who have not responded to standard treatments.

  Recruiting Phase 1/2 Henan Cancer Hospital · Zhengzhou, Henan · NCT07559097

• Methotrexate in hand osteoarthritis that hasn’t improved with usual treatments

  This study tests whether methotrexate injections can help people with erosive hand OA who haven’t benefited from standard care, compared with placebo.

  Recruiting Phase 2/3 Centre Hospitalier Universitaire de Nice · Nice · NCT07501507

• Subcutaneous methotrexate for Chinese adults with rheumatoid arthritis

  This project will test how well and how safely subcutaneous methotrexate works for Chinese adults with rheumatoid arthritis, including people with lung or coronary comorbidities.

  Recruiting Peking University People's Hospital · Beijing, Beijing Municipality · NCT07463937

• Repinatrabit's effects on common medicines like birth control, metformin, carbamazepine, rosuvastatin, and methotrexate

  This trial will test whether repinatrabit changes how common medicines—ethinyl estradiol/norethindrone (birth control), metformin, carbamazepine, rosuvastatin, and methotrexate—act in healthy adults.

  Recruiting Phase 1 Otsuka Pharmaceutical Development & Commercialization, Inc. · Melbourne, Victoria · NCT07446400

• PD‑1 blocker with rituximab, high‑dose methotrexate, and orelabrutinib for newly diagnosed primary or secondary CNS lymphoma

  This trial will test whether combining a PD‑1 blocker with rituximab, high‑dose methotrexate, and orelabrutinib helps people with newly diagnosed primary or secondary central nervous system diffuse large B‑cell lymphoma.

  Recruiting Phase 2 The First Affiliated Hospital with Nanjing Medical University · Nanjing, Jiangsu · NCT07410520

• Trying different doses of ELV001 for adults with active rheumatoid arthritis who didn't respond to methotrexate and TNF blockers.

  This trial will test whether different doses of ELV001 help adults with active rheumatoid arthritis who have not had enough benefit from methotrexate and TNF inhibitors.

  Recruiting at 34 sites Phase 2 Elevara Medicines Limited · Gilbert, Arizona and 33 other locations · NCT07409103

• Intravitreal ADX-2191 (methotrexate) injections for primary vitreoretinal lymphoma

  This trial tests whether different schedules of intravitreal ADX-2191 (methotrexate) injections are safe and active for adults with biopsy-proven primary vitreoretinal lymphoma.

  Recruiting Phase 3 Aldeyra Therapeutics, Inc. · Miami, Florida · NCT07402876

• Comparing subcutaneous versus intravenous pegloticase with methotrexate for uncontrolled gout

  This test compares subcutaneous pegloticase plus methotrexate to intravenous pegloticase plus methotrexate to see which better keeps uric acid under control in adults with uncontrolled gout.

  Recruiting at 75 sites Phase 3 Amgen · Birmingham, Alabama and 74 other locations · NCT07388498

• Cyclosporine versus methotrexate for children with alopecia areata

  This project tests whether cyclosporine or methotrexate works better as the first systemic treatment for children and teens (ages 2–17) with moderate-to-severe alopecia areata.

  Recruiting Phase 4 Erasmus Medical Center · Rotterdam · NCT07381556

• Methotrexate treatment for severe vulvar lichen sclerosus

  This trial will try methotrexate to reduce symptoms and prevent further scarring in women with severe vulvar lichen sclerosus who have not improved with high‑potency topical steroids.

  Recruiting Phase 2 Clinica Dermatologica Arbache ltda · São José dos Campos, São Paulo · NCT07352917

• Testing a four-drug regimen (methotrexate, rituximab, sintilimab, pirtobrutinib) versus investigator-selected care for newly diagnosed PCNSL

  This trial will try a combination of methotrexate, rituximab, sintilimab and pirtobrutinib as first treatment for adults with newly diagnosed primary central nervous system lymphoma to see if it increases complete remissions and is safe compared with standard doctor-selected treatments.

  Recruiting at 3 sites Phase 2 Tongji Hospital · Xiamen, Fujian and 3 other locations · NCT07350850

• Apremilast versus methotrexate for moderate to severe plaque psoriasis

  This test compares apremilast and methotrexate to see which works better for adults (18–60) with moderate to severe plaque psoriasis who have not had systemic therapy in the past 3 months.

  Recruiting Early Phase 1 Hayat Abad Medical Complex, Peshawar · Peshawar, Khyber Pukhtunkhwa · NCT07337434

• IV PRX-115 at different dose schedules, with or without methotrexate, versus placebo for adults with gout

  This trial will test whether IV PRX-115 given on different schedules, with or without low-dose methotrexate, can lower uric acid and be safe in adults whose gout is not controlled by standard urate-lowering drugs.

  Recruiting at 35 sites Phase 2 Protalix · Glendale, Arizona and 34 other locations · NCT07280156

• Oral methotrexate versus tofacitinib for chronic plaque psoriasis

  This trial will test whether oral methotrexate or tofacitinib works better for adults with moderate-to-severe chronic plaque psoriasis.

  Recruiting Early Phase 1 Lahore General Hospital · Lahore, Punjab Province · NCT07261306

• Discontinuing Methotrexate for 2 Weeks After PCV20 to See If Immunity Improves

  This trial tests whether stopping methotrexate for two weeks after the PCV20 vaccine helps adults with autoimmune rheumatic diseases develop a stronger immune response.

  Recruiting Phase 4 University of Sao Paulo General Hospital · São Paulo, São Paulo · NCT07242092

• Orelabrutinib and selinexor with methotrexate, thiotepa, or temozolomide for relapsed or refractory CNS lymphoma

  This trial will try adding oral orelabrutinib and selinexor to methotrexate (or thiotepa if MTX can't be used, or temozolomide) to see if it helps people with relapsed or refractory primary or secondary CNS lymphoma.

  Recruiting Ruijin Hospital · Shanghai, Shanghai Municipality · NCT07191431

• Methotrexate for ectopic pregnancy in patients with relative contraindications

  This review will see if methotrexate worked safely and effectively for women with ectopic pregnancy who had conditions that make the drug relatively risky.

  Recruiting Bagcilar Training and Research Hospital · Istanbul, Türkiye · NCT07186127

• Orelabrutinib Combined With Teniposide, Rituximab and Methotrexate for Newly Diagnosed PCNSL

  In this study, adults with newly diagnosed primary CNS lymphoma try different induction regimens to see which one works best before consolidation therapy.

  Recruiting at 9 sites Phase 2/3 Huashan Hospital · Beijing and 14 other locations · NCT07185373

• Probiotics plus methotrexate in early psoriasis

  This test will see if taking daily probiotics along with weekly methotrexate helps adults with newly diagnosed psoriasis have better skin outcomes and healthier gut bacteria than taking methotrexate alone.

  Recruiting Early Phase 1 Alexandria University · Alexandria · NCT07169019

• Methotrexate for knee osteoarthritis with ultrasound-proven synovitis

  This randomized trial will try methotrexate versus placebo to see if it reduces pain and inflamed lining (synovitis) in people aged 40–70 with knee osteoarthritis confirmed by ultrasound.

  Recruiting Phase 2 Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh · Dhaka, Dhaka Division · NCT07161336

• TRAF3IP2 gene variation (rs13190932) in people with psoriasis and psoriatic arthritis

  This trial will test whether the TRAF3IP2 (rs13190932) gene variant changes how adults with psoriasis or psoriatic arthritis respond to methotrexate treatment.

  Recruiting South Valley University · Qina · NCT07086924

• Selinexor with high-dose methotrexate, rituximab, and low-dose whole-brain radiotherapy for newly diagnosed CNS lymphoma patients who can't have transplant

  We will test whether adding oral selinexor to high-dose methotrexate and rituximab followed by low-dose whole-brain radiotherapy can help people with newly diagnosed central nervous system lymphoma who are not candidates for stem cell transplant.

  Recruiting Phase 2 Second Affiliated Hospital of Soochow University · Suzhou · NCT07002099

• Methotrexate with erlotinib and celecoxib for recurrent or metastatic head and neck cancer in rural Midwest patients

  This trial tests whether combining methotrexate, erlotinib, and celecoxib can help adults in the rural Midwest whose head and neck cancer has returned or spread.

  Recruiting Phase 2 Mayo Clinic · Rochester, Minnesota · NCT06997068

• ZR-MTX combo for newly diagnosed primary intraocular lymphoma

  This trial tests a Rituximab, Zanubrutinib, and methotrexate cocktail plus intravitreal methotrexate in people newly diagnosed with primary intraocular lymphoma to see if it can extend the time before the disease progresses.

  Recruiting Phase 2 Peking Union Medical College Hospital · Beijing, Beijing Municipality · NCT06973811

• Combining hydroxychloroquine with chemotherapy for advanced colorectal cancer

  This study is testing if adding hydroxychloroquine to standard chemotherapy can help people with advanced colorectal cancer live longer and feel better.

  Recruiting Phase 2 Pavlov First Saint Petersburg State Medical University · Saint Petersburg, Sankt-Peterburg · NCT06949982

• New treatment approach for primary central nervous system lymphoma

  This study is testing a new combination of treatments for people with newly diagnosed primary central nervous system lymphoma to see if it can help them respond better and stay healthy longer.

  Recruiting The First Hospital of Jilin University · Changchun · NCT06946407

• Evaluating Baricitinib for Skin Tightening in Systemic Sclerosis

  This study is testing if a new medication called baricitinib can help improve skin tightness in people with diffuse cutaneous systemic sclerosis compared to a standard treatment.

  Recruiting Phase 3 Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh · Dhaka · NCT06936215

• Measuring Methotrexate Levels in Rheumatoid Arthritis Patients

  This study is testing how methotrexate levels change in people with rheumatoid arthritis when they switch from taking it by mouth to getting it as an injection, and how these levels relate to the treatment's effectiveness and safety.

  Recruiting Phase 4 Keio University · Shinjuku-ku, Tokyo · NCT06913907

• Spinal injections of cytarabine, methotrexate, and hydrocortisone to prevent severe CAR T neurotoxicity

  This phase II test tries whether giving intrathecal cytarabine, methotrexate, and hydrocortisone around day 4 (with a day 7 hydrocortisone if no symptoms) can prevent severe neurotoxicity in adults receiving Yescarta or Tecartus CAR T-cell therapy.

  Recruiting Phase 2 OHSU Knight Cancer Institute · Portland, Oregon · NCT06895473

• Investigating a genetic polymorphism in Egyptian patients with psoriatic arthritis

  This study is testing if a specific gene variation affects how Egyptian patients with psoriatic arthritis respond to treatment with methotrexate compared to a placebo.

  Recruiting South Valley University · Qina · NCT06870500

• Using RMTT regimen as first-line therapy for primary central nervous system lymphoma

  This study is testing if a new combination treatment called RMTT can help people with primary central nervous system lymphoma who haven't had treatment before.

  Recruiting Zhejiang Cancer Hospital · Hangzhou, Zhejiang · NCT06832267

• Zanubrutinib combined with chemotherapy for patients with secondary CNS lymphoma

  This study is testing if a new combination of medications, including Zanubrutinib and chemotherapy, can help people with secondary Central Nervous System lymphoma feel better and improve their health.

  Recruiting Phase 1 University of Miami · Miami, Florida · NCT06730542

• Evaluating the effects of oral AP1189 in early rheumatoid arthritis patients

  This study is testing if a new oral medication called AP1189 can help people with early rheumatoid arthritis feel better when taken with methotrexate.

  Recruiting at 11 sites Phase 2 SynAct Pharma Aps · Cutler Bay, Florida and 10 other locations · NCT06671054

• Optimizing methotrexate therapy for rheumatoid arthritis patients

  This study is testing whether giving methotrexate as a pill or an injection helps people with active rheumatoid arthritis feel better and respond to treatment more effectively.

  Recruiting Phase 4 Medical University of Vienna · Vienna, State of Vienna · NCT06649136

• Comparing two treatment regimens for Graft-versus-host Disease after stem cell transplantation

  This study is testing two different treatment plans for adults with Graft-versus-host Disease after stem cell transplantation to see which one works better, especially for those who haven't responded to steroids.

  Recruiting at 30 sites Phase 3 Incyte Corporation · Palo Alto, California and 29 other locations · NCT06615050

• Treatment for newly diagnosed primary central nervous system lymphoma using pomadomide, rituximab, and methotrexate

  This study is testing a new combination of medications, including pomadomide, rituximab, and methotrexate, to see if it can safely help people with newly diagnosed primary central nervous system lymphoma.

  Recruiting Phase 1/2 Second Affiliated Hospital, Zhejiang University, School of Medicine · Hanzhou, Zhejiang · NCT06554561

• Using Thiotepa and Methotrexate for Breast Cancer with Leptomeningeal Metastasis

  This study is testing if a combination of thiotepa and methotrexate can help people with breast cancer that has spread to the protective layers around the brain feel better and live longer.

  Recruiting Phase 2 The First Affiliated Hospital with Nanjing Medical University · Nanjing, Jiangsu · NCT06543992

• Preventing vision loss after retinal detachment surgery with methotrexate

  This study is testing if a medication called methotrexate can help prevent vision loss from complications after retinal detachment surgery in adults.

  Recruiting Phase 2 Cincinnati Eye Institute, Southwest Ohio · Cincinnati, Ohio · NCT06541574

• Two-step ATG to prevent GVHD after myeloablative allogeneic stem cell transplant

  This study tests whether a two-step dosing of rabbit ATG added to tacrolimus and low-dose methotrexate can help prevent severe acute and chronic GVHD in adults 18–60 receiving myeloablative allogeneic peripheral blood stem cell transplants for leukemia, MDS, or myeloproliferative disorders.

  Recruiting Phase 2 University of Alabama at Birmingham · Birmingham, Alabama · NCT06315309

• Methotrexate plus tofacitinib for rheumatoid arthritis

  This project will test whether adding tofacitinib to methotrexate helps adults with early, moderate-to-severe rheumatoid arthritis who have a myeloid-stromal synovial pattern.

  Recruiting at 5 sites Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University · Foshan, Guangdong and 4 other locations · NCT06301373

• Using Peguricase with Methotrexate for Uncontrolled Gout

  This study is testing if combining an injection of peguricase with methotrexate can help people with uncontrolled gout who haven't found relief with standard treatments.

  Recruiting Phase 1 Shanghai Institute Of Biological Products · Bengbu, Anhui · NCT06298071

• Preventing Acute GVHD After Stem Cell Transplant

  This study is testing a new way to give a medication before and during stem cell transplants to see if it can help prevent a serious condition called acute GVHD in patients.

  Recruiting Phase 2 University of Alabama at Birmingham · Birmingham, Alabama · NCT06265584

• Testing a new approach to prevent graft-versus-host disease after stem cell transplantation

  This study is testing a new treatment to see if a combination of two drugs can help prevent graft-versus-host disease in patients receiving stem cell transplants, allowing them to stop taking immunosuppressive drugs sooner.

  Recruiting at 3 sites Phase 2 Nantes University Hospital · Angers and 2 other locations · NCT06252870

• Comparing Methotrexate and Placebo for Treating Atopic Dermatitis in Adults

  This study tests if methotrexate can help adults with moderate to severe atopic dermatitis feel better compared to a placebo when other treatments haven't worked.

  Recruiting at 20 sites Phase 3 medac GmbH · Prague, Praha 1 and 29 other locations · NCT06239311

• Using ruxolitinib, tacrolimus, and methotrexate to prevent graft versus host disease in young patients after bone marrow transplant

  This study is testing if a combination of three medications can help prevent graft versus host disease in young patients after they receive a bone marrow transplant for certain types of leukemia or blood disorders.

  Recruiting Phase 2 City of Hope Medical Center · Duarte, California · NCT06128070

• Phase II trial of CMC regimen for early stage breast cancer

  This study is testing a new combination of oral medications for people with early-stage breast cancer to see if it helps them manage their condition safely and effectively.

  Recruiting Phase 2 University of Illinois Chicago · Chicago, Illinois · NCT06085742

• MRG003 vs cetuximab/methotrexate for RM-SCCHN after prior therapies

  This trial tests whether MRG003 can help people with recurrent/metastatic squamous cell carcinoma of the head and neck who have already tried PD-1 inhibitors and platinum therapy by comparing it to cetuximab plus methotrexate as a later-line treatment.

  Recruiting Phase 3 Lepu Biopharma Co., Ltd. · Shanghai, Shanghai Municipality · NCT05751512

• Combination treatment for relapse refractory central nervous system lymphoma

  This study is testing a new combination of three medications to see if it can help people with hard-to-treat central nervous system lymphoma feel better.

  Recruiting Phase 1/2 Huashan Hospital · Hefei, Anhui and 4 other locations · NCT05698147

• Combination treatment for newly diagnosed primary central nervous system lymphoma

  This study is testing a new combination of treatments for people with newly diagnosed primary central nervous system lymphoma to see if it helps them respond better and live longer.

  Recruiting Phase 2 Second Affiliated Hospital, Zhejiang University, School of Medicine · Hanzhou, Zhejiang · NCT05600660

• Combination treatment for patients with primary central nervous system lymphoma

  This study is testing a new combination of treatments for adults with primary central nervous system lymphoma who can't have high-dose chemotherapy to see if it helps them get better and live longer.

  Recruiting Phase 2 University of Cologne · Stuttgart, Baden-Wurttemberg and 5 other locations · NCT05583071

• Lenalidomide maintenance for patients with primary CNS lymphoma

  This study is testing if lenalidomide can help patients with primary CNS lymphoma stay in remission after their initial treatment.

  Recruiting Phase 2 Seoul National University Bundang Hospital · Seongnam-si, Please Select · NCT05260619

• Combining R-CDOP and intrathecal methotrexate for high-risk DLBCL patients

  This study is testing a new combination of chemotherapy and a specific type of treatment for high-risk patients with untreated diffuse large B-cell lymphoma to see if it helps prevent serious complications in the brain.

  Recruiting Phase 2 Fudan University · Shanghai, Shanghai Municipality and 1 other locations · NCT05257018

• Comparing Azathioprine and Methotrexate with Adalimumab for Crohn's Disease

  This study is testing whether adding azathioprine or methotrexate to adalimumab helps people with Crohn's disease who haven't improved with other treatments.

  Recruiting Phase 3 Centre Hospitalier Universitaire, Amiens · Amiens · NCT05040464

• Combination treatment for primary central nervous system lymphoma

  This study is testing a new combination of medications to see if it can help people with primary central nervous system lymphoma feel better and improve their treatment outcomes.

  Recruiting Phase 2 Henan Cancer Hospital · Zhengzhou, Henan · NCT04737889

• Using Avelumab and Methotrexate to Treat Low-risk Gestational Trophoblastic Neoplasias

  This study is testing if combining avelumab with methotrexate can help women with low-risk gestational trophoblastic neoplasias feel better and have fewer side effects than standard chemotherapy.

  Recruiting at 6 sites Phase 1/2 Hospices Civils de Lyon · Pierre-Bénite, Pierre Bénite and 8 other locations · NCT04396223

• Comparing two methods to prevent GVHD in leukemia patients after transplant

  This study is testing whether a new method using cyclophosphamide can better prevent graft-versus-host disease in leukemia patients after they receive a stem cell transplant compared to the usual treatment.

  Recruiting Phase 3 King Faisal Specialist Hospital & Research Center · Riyadh · NCT04314219

• Combining blinatumomab, methotrexate, cytarabine, and ponatinib for treating certain types of leukemia

  This study is testing a new combination of four medications to see if it can help people with certain types of leukemia that have come back or didn't respond to previous treatments.

  Recruiting Phase 2 M.D. Anderson Cancer Center · Houston, Texas · NCT03263572

• Comparing targeted therapy with triple therapy for rheumatoid arthritis patients not responding to standard treatments

  This study is testing whether adding a targeted therapy to existing medications works better than using a combination of traditional drugs for people with rheumatoid arthritis who haven't felt better with standard treatments.

  Recruiting Phase 4 University Hospital, Strasbourg, France · Strasbourg · NCT02714634

• Evaluating high-dose methotrexate for breast cancer with leptomeningeal metastasis

  This study is testing if high-dose methotrexate can help breast cancer patients with leptomeningeal disease feel better and improve their treatment options.

  Recruiting at 2 sites Phase 2 Wake Forest University Health Sciences · Baltimore, Maryland and 2 other locations · NCT02422641

Quick answers

How many clinical trials of Methotrexate are recruiting right now?

62, as of November 12, 2026. We update this list each time we refresh our data from ClinicalTrials.gov.

Where are these trials running?

In 27 countries. The most sites are in China (21 trials), United States (17 trials), France (6 trials), Egypt (3 trials), Bangladesh (2 trials). Each study page lists its sites and which ones are recruiting.

What phases are they in?

Phase 2: 28, Phase 3: 9, Phase 4: 5, Phase 1/2: 4. Trials with no phase listed are usually device, behavioural or observational studies.

How do I join one?

Open a study, check the "Is this trial a fit for you?" section, then contact the study team listed on the page. They will ask a few screening questions. Asking is free and does not commit you to anything. Talk it over with your doctor first.

Find the studies that fit you

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