Effects of Dairy Fat and Fermentation on Gut Microbiome and Heart Disease Risk

Effect of Full-Fat and Fat-Free Dairy, With and Without Fermentation, on Fecal Microbiome, Fecal and Serum Metabolome, and Host Cardiometabolic Risk Status

Not applicable Interventional Tufts University · NCT05840081

This study is testing whether eating full-fat or fat-free dairy products affects gut health and heart disease risk in people over 50.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment20 (estimated)
Ages50 Years and up
SexAll
SponsorTufts University Academic / other
Locations1 site (Boston, Massachusetts)
Trial IDNCT05840081 on ClinicalTrials.gov

What this trial studies

This study investigates how consuming full-fat versus fat-free milk and yogurt affects gut microorganisms and their metabolic products, as well as heart disease risk factors. Participants over 50 years old will follow a randomized-controlled cross-over design, consuming two servings of dairy products daily across four diet phases, each lasting three weeks. The study will measure various health metrics, including weight, waist circumference, blood pressure, and collect blood and stool samples to analyze changes in the gut microbiome and cardiometabolic risk factors. The findings aim to refine dietary guidelines for reducing cardiovascular disease risk.

Who should consider this trial

Good fit: Ideal candidates are men and postmenopausal women over 50 years old with a BMI between 25 and 35 who consume dairy products.

Not a fit: Patients under 50 years old, pre- or peri-menopausal women, or those with certain health conditions or dietary restrictions may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could provide insights into how dairy fat content and fermentation influence heart disease risk, potentially leading to improved dietary recommendations.

How similar studies have performed: While studies on dairy consumption and heart disease exist, this specific approach comparing fat content and fermentation effects on gut microbiota is relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

1. Age \>50 years
2. Men and postmenopausal women
3. BMI \>25 and \<35 kg/m2
4. No milk allergy, lactose intolerance or adherence to vegan or other dietary pattern that excludes dairy products
5. Non-smoker
6. Usual pattern of bowel movements at least every other day.

Exclusion Criteria:

1. Women and men \<50 years
2. Women, pre- or peri-menopausal
3. BMI \<25 and \>35 kg/m2
4. Current smokers or former smoker who quit \< 6 months
5. Use of nicotine replacement products within last 6 months
6. Replacement or gender affirming hormonal therapy use
7. Weight gain of \>15 pounds within last 6 months
8. Infrequent bowel movements
9. History of anemia within past 2 years or confirmation of anemia at first study visit
10. Blood donation within last 2 months
11. Untreated hypertension
12. Oral antibiotics use within 2 weeks of study entry
13. Steroid use (including dental prophylaxis use within 2 wks prior to or during study, except non-prescription topical and nasal steroids, e. g. Flonase)
14. Use of lipid lowering medications
15. Chronic use of aspirin, non-steroidal anti-inflammatory medications (NSAIDS), laxatives, anti-diarrheal medication
16. Regular use (\>2 times per week) of acid lowering medication: antacids, proton pump inhibitors, or H2 Blockers.
17. Use of dietary supplements other than multi-vitamins, including pre- and probiotics, within 2 months of study entry
18. Milk allergy, lactose intolerance or adherence to vegan or other dietary patterns that exclude dairy products
19. Colonoscopy within 3 weeks of study entry or during study period
20. Diagnosis of malabsorption, inflammatory bowel disease (ulcerative colitis or Crohn's Disease), cirrhosis, history of gastrointestinal resection (other than appendectomy), chronic pancreatitis or any other chronic GI disease that in the opinion of the study physician would alter the study results
21. Chronic heart, liver, thyroid, renal or kidney disease
22. Type I or type II diabetes
23. Alcohol consumption \>7 drinks/week for women and \>14 drinks/week for men
24. Inadequate refrigerator storage capacity to store 21 8-oz containers of milk or yogurt
25. Participation in other dietary intervention research study during the same time
26. No social security number
27. Not willing to commit to adhering to the protocol

Where this trial is running

Boston, Massachusetts

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Heart DiseasesMicrobial ColonizationInflammationgut microbiomegut microbiotayogurtmilkheart disease
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.