CAR-γδT cell therapy targeting GPC3 and Mesothelin for cancer treatment

GPC3/Mesothelin Targeted CAR-γδT for Immunotherapy of Solid Cancer: Phase I Clinical Trial

Phase 1 Interventional Second Affiliated Hospital of Guangzhou Medical University · NCT06196294

This study is testing a new cancer treatment that uses modified immune cells to target specific proteins in patients with advanced solid tumors.

Quick facts

PhasePhase 1
Study typeInterventional
Enrollment30 (estimated)
Ages18 Years to 75 Years
SexAll
SponsorSecond Affiliated Hospital of Guangzhou Medical University Academic / other
Drugs / interventionsprednisone, immunotherapy
Locations1 site (Guangzhou, Guangdong)
Trial IDNCT06196294 on ClinicalTrials.gov

What this trial studies

This phase 1 clinical trial evaluates the safety, tolerance, and preliminary efficacy of GPC3/Mesothelin-CAR-γδT cell immunotherapy in patients with advanced solid tumors expressing GPC3 or Mesothelin. Patients will undergo biopsy to confirm protein expression, followed by the isolation and transfection of their γδT cells with CAR targeting these proteins. The modified cells will be reintroduced into the patients, potentially alongside immune checkpoint inhibitors, to enhance their anti-cancer activity. The study aims to gather data on the clinical outcomes and safety of this innovative treatment approach.

Who should consider this trial

Good fit: Ideal candidates are patients with advanced cancers that express GPC3 or Mesothelin and have a life expectancy of more than 12 weeks.

Not a fit: Patients with prior gene therapy, severe viral infections, or other significant health issues may not benefit from this study.

Why it matters

Potential benefit: If successful, this therapy could provide a novel and effective treatment option for patients with advanced cancers that express GPC3 or Mesothelin.

How similar studies have performed: While CAR-T cell therapies have shown success in various cancers, this specific approach using CAR-γδT cells targeting GPC3 and Mesothelin is novel and has not been extensively tested in clinical settings.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

1\. Patients with advanced cancer that expresses GPC3 or Mesothelin protein; 2. Life expectancy \>12 weeks; 3. Adequate heart, lung, liver, kidney, and blood function; 4. Available autologous transduced T cells with greater than or equal to 20% expression of GPC3/Mesothelin-CAR determined by flow-cytometry and killing of Mesothelin-positive targets greater than or equal to 20% in cytotoxicity assay; 5. Informed consent explained to, understood by and signed by patient/guardian. Patient/guardian given copy of informed consent.

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Exclusion Criteria:

1. Had accepted gene therapy before;
2. Severe virus infection such as HBV, HCV, HIV, et al;
3. Known HIV positivity;
4. Active infectious disease related to bacteria, virus,fungi,et al;
5. Other severe diseases that the investigators consider not appropriate;
6. Pregnant or lactating women;
7. Systemic steroid treatment (greater than or equal to 0.5 mg prednisone equivalent/kg/day);
8. Other conditions that the investigators consider not appropriate. -

Where this trial is running

Guangzhou, Guangdong

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Pancreas CancerLung CancerLiver CancerMesotheliomaCAR-T Cell TherapySolid Tumor, AdultSolid TumorCAR-γδT
Last reviewed 2026-06-10 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.