Using washed microbiota transplantation to treat autism spectrum disorder

Washed Microbiota Transplantation (WMT) for Autism Spectrum Disorder (ASD)

Phase 1 Interventional The Second Hospital of Nanjing Medical University · NCT06030752

This study is testing whether a new treatment using healthy gut bacteria can help improve symptoms in children with Autism Spectrum Disorder.

Quick facts

PhasePhase 1
Study typeInterventional
Enrollment80 (estimated)
Ages3 Years to 17 Years
SexAll
SponsorThe Second Hospital of Nanjing Medical University Academic / other
Locations2 sites (Nanjing, Jiangsu and 1 other locations)
Trial IDNCT06030752 on ClinicalTrials.gov

What this trial studies

This study explores the use of washed microbiota transplantation (WMT) to improve symptoms in children diagnosed with Autism Spectrum Disorder (ASD). It focuses on the gut-brain-microbiota axis, investigating how intestinal microbes can influence brain function. Participants aged 3 to 14 years who meet specific diagnostic criteria will receive repeated WMT from healthy donors, with microbiota analysis conducted before and after the procedure. The study aims to evaluate both the efficacy and safety of this innovative approach in treating ASD symptoms.

Who should consider this trial

Good fit: Ideal candidates are children aged 3 to 14 years who meet the DSM-5 diagnostic criteria for autism spectrum disorder.

Not a fit: Patients with severe comorbidities, gastrointestinal diseases, or those who have used antibiotics or probiotics in the last three months may not benefit from this study.

Why it matters

Potential benefit: If successful, this approach could provide a novel treatment option that improves symptoms of autism spectrum disorder in children.

How similar studies have performed: While few studies have explored microbiota transplantation for ASD, this approach is relatively novel and has not been widely tested in clinical settings.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Children and adolescents with an established diagnosis of ADHD or ADHD + ASD according to the Diagnostic and Statistical Manual Of Mental Disorders Fifth Edition;.
* Age 3-17 years.
* Received stable treatments for ≥1 month preceding WMT

Exclusion Criteria:

* Their guardian could not understand the questionnaires or provide informed consent.
* Diagnosed with a single-gene disorder, major brain malformations, gastrointestinal diseases (ulcerative colitis, Crohn's disease, or eosinophilic esophagitis)
* Had severe comorbidities including cardiopulmonary failure, severe liver, and kidney diseases, severe infection, tumors, etc.
* Accompanied with other life-threatening disorders required emergency treatment.
* Unable to tolerate colonoscopy or anesthesia.

Where this trial is running

Nanjing, Jiangsu and 1 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Autism Spectrum Disorderfecal Microbiota TransplantationAutism spectrum disorderGut microbiotaGut-brain axis
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.