Using fat-derived stem cells to treat spinal cord injuries

CELLTOP Part II: A Phase II Clinical Trial of Autologous Adipose Derived Mesenchymal Stem Cells in the Treatment of Paralysis Due to Traumatic Spinal Cord Injury

Phase 2 Interventional Mayo Clinic · NCT04520373

This study is testing whether injecting fat-derived stem cells into the spinal canal can help people with spinal cord injuries recover and improve their function.

Quick facts

PhasePhase 2
Study typeInterventional
Enrollment40 (estimated)
Ages18 Years and up
SexAll
SponsorMayo Clinic Academic / other
Drugs / interventionsmethotrexate
Locations1 site (Rochester, Minnesota)
Trial IDNCT04520373 on ClinicalTrials.gov

What this trial studies

This study investigates the safety and potential therapeutic effects of injecting autologous, culture-expanded, adipose-derived mesenchymal stem cells into the spinal canal of patients with spinal cord injuries. Participants will receive these stem cell injections alongside best medical management, which includes occupational and physical therapy. The goal is to assess whether this innovative approach can improve recovery and function in individuals suffering from paralysis due to spinal cord injuries.

Who should consider this trial

Good fit: Ideal candidates are adults aged 18 and older with spinal cord injuries who meet specific inclusion criteria.

Not a fit: Patients with conditions unrelated to spinal cord injuries or those who do not meet the eligibility criteria may not benefit from this study.

Why it matters

Potential benefit: If successful, this treatment could significantly enhance recovery and quality of life for patients with spinal cord injuries.

How similar studies have performed: Other studies using stem cell therapies for spinal cord injuries have shown promise, indicating potential for success in this approach.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Male or female aged 18 years and older

  * Females of childbearing potential must have a negative pregnancy test prior to receiving the study drug and will agree to use adequate contraception (hormonal/barrier method or abstinence) from the time of screening to a period of 1 year following completion of the drug treatment cycle. Females of childbearing potential are defined as premenopausal and not surgically sterilized, or post-menopausal for fewer than 2 years. If the urine pregnancy test is positive, the study drug will not be administered and the result will be confirmed by a serum pregnancy test. Serum pregnancy tests will be performed at a central clinical laboratory, whereas urine pregnancy tests will be performed by qualified personnel using kit.
  * Females becoming pregnant during the study will continue to be monitored for the duration of the study or completion of the pregnancy, whichever is longer. Monitoring will include perinatal and neonatal outcome. Any SAEs associated with pregnancy will be recorded.
* AIS grade A or B of SCI at the time of injury with or without subsequent improvement within 1 year of injury that has progressed to a higher AIS grade with a plateau in functional improvement
* SCI must be traumatic, blunt/non-penetrating in nature and not degenerative
* Full understanding of the requirements of the study and willingness to comply with the treatment plan, including fat harvesting, laboratory tests, diagnostic imaging, complete physical and neurologic examination and follow-up visits and assessments
* Full understanding of the requirements of home exercise program prescribed by physical and occupational therapists.
* Once the nature of the study is fully explained and prior to any study-related procedure is initiated the subject is willing to provide written, informed consent and complete HIPAA documentation

Exclusion Criteria:

* Pregnant or nursing, or planning on becoming pregnant during the study period
* AIS grade of SCI other than A or B at the time of injury
* Non-traumatic SCI
* History of receiving mesenchymal stem cell, gene or exosome therapy for any indications
* History of intra-spinal infection
* History of superficial infection in the index spinal level within 6 months of study
* Evidence of current superficial infection affecting the index spinal level at the time of enrollment
* On chronic, immunosuppressive transplant therapy or having a chronic, immunosuppressive state, including use of systemic steroids/corticosteroids
* Taking anti-rheumatic disease medication (including methotrexate or other antimetabolites) within 3 months prior to study enrollment
* Ongoing infectious disease, including but not limited to tuberculosis, HIV, hepatitis, and syphilis
* Fever, defined as temperature above 100.4 F/38.0 Celsius, or mental confusion at baseline
* Significant improvement between the time of adipose tissue harvest and the time of injection, defined as improvement from AIS grade A or B to AIS grade C or greater.
* Clinically significant cardiovascular (e.g. history of myocardial infarction, congestive heart failure or uncontrolled hypertension \> 90 mmHg diastolic and/or 180 mmHg systolic), neurological (e.g. stroke, TIA) renal, hepatic or endocrine disease (e.g. diabetes, osteoporosis).
* History of malignancy including melanoma with the exception of localized skin cancers (with no evidence of metastasis, significant invasion, or re-occurrence within three years of baseline). Any other malignancy will not be allowed.
* History of blood dyscrasia, including but not limited to anemia, thrombocytopenia, and monoclonal gammopathy
* Participation in a study of an experimental drug or medical device within 3 months of study enrollment
* Known allergy to local anesthetics of other components of the study drug
* Any contraindication to MRI scan according to MRI guidelines, or unwillingness to undergo MRI procedures
* History of or current evidence of alcohol or drug abuse or dependence, recreational use of illicit drug or prescription medications, or use of medical marijuana within 30 days of study entry
* Diagnosis of schizophrenia or bipolar disorder
* Patients with baseline depression, diagnosed by the Beck Depression Inventory Assessment
* Currently taking riluzole for treatment of amyotrophic lateral sclerosis (ALS)

Where this trial is running

Rochester, Minnesota

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Spinal Cord InjuriesParalysis
Last reviewed 2026-06-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.