Transplanting engineered stem cells into the brain for ALS treatment

Human Neural Progenitor Cells Secreting Glial Cell Line-Derived Neurotrophic Factor (CNS10-NPC-GDNF) Delivered to the Motor Cortex for the Treatment of Amyotrophic Lateral Sclerosis

Phase 1 Interventional Cedars-Sinai Medical Center · NCT05306457

This study is testing if transplanting specially modified stem cells into the brains of people with ALS is safe and could help improve their condition.

Quick facts

PhasePhase 1
Study typeInterventional
Enrollment16 (estimated)
Ages18 Years and up
SexAll
SponsorCedars-Sinai Medical Center Academic / other
Locations1 site (Los Angeles, California)
Trial IDNCT05306457 on ClinicalTrials.gov

What this trial studies

This study investigates the safety of transplanting genetically modified neural progenitor cells that produce a growth factor called GDNF into the motor cortex of patients with Amyotrophic Lateral Sclerosis (ALS). It is a Phase 1/2a, single-center trial that will assess two escalating doses of these cells delivered to the brain's 'hand-knob' area. Participants will be enrolled sequentially, with a minimum interval between surgeries to monitor safety and adverse events. The study aims to determine if this novel approach can be safely implemented in humans, as it has only been tested in animals previously.

Who should consider this trial

Good fit: Ideal candidates include adults aged 18 and older with a confirmed diagnosis of ALS and progressive weakness in upper extremities.

Not a fit: Patients with a longer duration of ALS symptoms or those currently taking riluzole or edaravone may not benefit from this study.

Why it matters

Potential benefit: If successful, this treatment could provide a new therapeutic option for patients with ALS, potentially slowing disease progression.

How similar studies have performed: While this approach is novel in humans, previous studies have shown promise in animal models using similar cell-based therapies.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion:

1. Confirmed diagnosis of ALS (Possible, Lab-supported Probable, Probable or Definite El Escorial Criteria)
2. Duration of ALS symptoms ≤ 36 months
3. Progressive weakness in upper extremities, with EMG supported evidence of denervation in both upper extremities
4. Forced Vital Capacity ≥50% of predicted normal in supine
5. Age: 18 years or older
6. Able to provide Informed Consent
7. Be geographically accessible to the study site and able to travel to study site for required visits
8. Have caregiver to assist in the transportation and care required by participation in the study
9. Not taking riluzole and/or edaravone or on a stable dose for ≥ 30 day
10. For women of child bearing capacity, negative pregnancy test prior to surgery and willingness to use birth control for the duration of the trial.
11. Medically able to undergo craniotomy as determined by the site PI and/or investigators
12. Medically able to tolerate the immunosuppression regimen as determined by the site PI

Exclusion:

1. Using invasive ventilatory assistance
2. Diagnosis of another active or unstable medical illness that may interfere with study participation at discretion of PI
3. Presence of any of the following conditions:

   1. Current drug or alcohol abuse
   2. Any known immunodeficiency syndrome
   3. Unstable medical condition
   4. Unstable psychiatric illness including psychosis and untreated major depression within 90 days of screening
4. Persons of child bearing capacity not willing to practice birth control
5. Receiving any investigational device/biologic/drug in the past 30 days or any previous exposure to stem cell therapy
6. Any condition in the upper extremities that precludes serial strength or coordination testing
7. Any condition that the investigators feel may pose complications for the surgery
8. Any condition or ALS disease phenotype that the site PI feels may interfere with participation in the study or in the interpretation of study endpoints
9. Allergy to Beta-Lactam antibiotics
10. Donor Specific Antibodies (DSA) ≥ 2500MFI or CPRA ≥ 20%
11. Contraindications to MRI

Where this trial is running

Los Angeles, California

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Amyotrophic Lateral SclerosisALSStem cellsGrowth FactorRegenerativeNeural Progenitor CellsNPCTransplantation
Last reviewed 2026-06-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.