Testing for specific mutations in metastatic breast cancer patients using liquid biopsy

Development and Validation of an Integrated Testing Strategy for Simultaneous Detection of ESR-1 and gBRCA Mutations Via Liquid Biopsy in HR+/HER2- Metastatic Breast Cancer (mBC) Patients, and the Creation of a Digital Gene Library to Support an Evidence-based Diagnostic Algorithm

Observational European Institute of Oncology · NCT06762483

This study is testing a new blood test to see if it can find specific gene mutations in people with certain types of metastatic breast cancer to help guide their treatment.

Quick facts

Study typeObservational
Enrollment80 (estimated)
Ages18 Years and up
SexFemale
SponsorEuropean Institute of Oncology Academic / other
Locations1 site (Milan, Italy)
Trial IDNCT06762483 on ClinicalTrials.gov

What this trial studies

This study aims to develop and validate a testing strategy that allows for the simultaneous detection of ESR-1 and gBRCA mutations in patients with hormone receptor-positive and HER2-negative metastatic breast cancer through liquid biopsy. The approach involves creating a digital gene library to support an evidence-based diagnostic algorithm, which could enhance understanding of treatment resistance mechanisms. Participants will include those with confirmed diagnoses of the specified breast cancer types, either before starting treatment for metastatic disease or after experiencing progression following prior treatments.

Who should consider this trial

Good fit: Ideal candidates are women diagnosed with HR+/HER2- metastatic breast cancer who have not yet started treatment or have progressed after treatment with CDK4/6 inhibitors.

Not a fit: Patients with non-HR+/HER2- breast cancer or those who do not have a confirmed diagnosis of breast cancer will not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to more effective personalized treatment strategies for patients with metastatic breast cancer.

How similar studies have performed: Other studies have shown promise in using liquid biopsy for mutation detection in various cancers, indicating that this approach may be effective.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Participants must have a confirmed diagnosis of estrogen receptor-positive (ER+) and/or progesterone receptor-positive (PgR+) breast cancer through histological and/or cytological examination by the local laboratory Participants must exhibit HER2-negative breast cancer

  1. or 2+). If IHC is 2+, a negative in situ hybridization (Fluorescent in situ hybridization (FISH), Chromogenic in situ hybridization (CISH), or Silver-enhanced in situ hybridization (SISH)) test is required by local laboratory testing.

     * Participants should be in an advanced or metastatic setting including both those prior to the initiation of treatment for metastatic disease and those who experienced progression following treatment with cyclin-dependent kinases (CDK)4/6 inhibitors. However, the primary tumor should be treated according to the standard of care.
     * Written informed consent must be signed and dated by the patient and the investigator prior to inclusion.

Exclusion Criteria:

* Unable to provide written informed consent

Where this trial is running

Milan, Italy

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Breast Cancer, MetastaticHR+/HER2- BRCA
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.