NKX019 therapy for patients with systemic lupus erythematosus

A Phase 1 Study of NKX019, a CD19 Chimeric Antigen Receptor Natural Killer (CAR NK) Cell Therapy, in Subjects With Systemic Lupus Erythematosus

This study is testing a new treatment called NKX019 to see if it can safely help people with systemic lupus erythematosus feel better.

Quick facts

• Phase: Phase 1
• Study type: Interventional
• Enrollment: 6 (estimated)
• Ages: 18 Years to 65 Years
• Sex: All
• Sponsor: Columbia University (Academic / other)
• Drugs / interventions: CAR-T, prednisone, cyclophosphamide
• Locations: 1 site (New York, New York)
• Trial ID: NCT06518668 on ClinicalTrials.gov

What this trial studies

This Phase 1 clinical trial evaluates the safety and tolerability of NKX019, a CD19 CAR NK cell therapy, in individuals diagnosed with systemic lupus erythematosus (SLE). Participants will undergo lymphodepletion with cyclophosphamide followed by NKX019 administration. The study includes four periods: Screening, Active Treatment, Follow-up, and Extended Follow-up, with disease assessments occurring every 90 days for two years. The trial aims to assess both the clinical activity of NKX019 and its pharmacokinetics and immunogenicity.

Who should consider this trial

Good fit: Ideal candidates are adults aged 18-65 with active systemic lupus erythematosus, particularly those with active lupus nephritis.

Not a fit: Patients with mild or inactive systemic lupus erythematosus or those outside the specified age range may not benefit from this study.

Why it matters

Potential benefit: If successful, this therapy could provide a new treatment option for patients suffering from systemic lupus erythematosus.

How similar studies have performed: While CAR T-cell therapies have shown promise in other conditions, this specific approach for systemic lupus erythematosus is novel and has not been extensively tested.

Eligibility criteria

Inclusion Criteria:

* Age: 18-65 years old at the time of informed consent
* Signed informed consent form, able to adhere to the study visit schedule, and comply with other requirements of the study as specified in the protocol
* Score of 8 or more points on the Hybrid-SLEDAI with at least 6 points from clinical items and at least one BILAG A or 2 B
* If SLE and LN: Active nephritis Class III or IV with or without Class V using the 2018 International Society of Nephrology and Renal Pathology Society (ISN/RPS) criteria as evidenced on kidney biopsy during screening or within 12 months before study enrollment. Per NIH indices, subjects must have at least activity score ≥ 2 and no more than moderate chronicity index. Subjects must have urinary protein:creatinine ratio (UPCR) ≥ 1.5 g/g or proteinuria ≥ 1.5 g/day, however, subjects must have ≤7g/ day of proteinuria.
* For patients with biopsies older than 6 months we will include an adjudication committee to review clinical data and decide on appropriateness of including the patient (see section 7.3). Additionally, all patients with active LN maximally tolerated doses of renin angiotensin aldosterone system (RAAS) blockade agents, except for patients with contraindications or intolerance to RAAS.
* Women of childbearing potential must have a negative serum pregnancy test at screening and agree to complete abstinence from intercourse from 2 weeks prior to administration of the first dose of study agent until 1 year after the last dose of study agents OR consistent and correct use of acceptable birth control (e.g. levonorgestrel implants, ethinyl estradiol/etonogestrel vaginal ring; injectable progesterone, intrauterine device \[IUD\] with failure rate \< 1% per year, oral contraceptives, double barrier method, transdermal contraceptive patch) OR male partner sterilization with documentation of azoospermia prior to subject's entry into the study.
* Male patients must agree to always use a latex or other synthetic condom during any sexual activity with women of childbearing potential. Male participants must agree to continue to use a condom during the intervention period and for at least 1 year following last treatment with investigational product. Female partners of male participants should be advised to use a highly effective method of contraception during treatment period and at least 12 months following treatment.
* One of the following: positive antinuclear antibodies (ANA) ≥ 1:80 at screening OR positive anti-dsDNA OR positive anti-Smith (anti-Sm) as determined by the local laboratory.
* Refractory SLE defined as having received ≥ 2 prior therapies for SLE (one immunosuppressant and one biologic/advanced therapy) and had an inadequate response to therapy despite being on a therapeutic dose for ≥ 90 days.
* For subjects taking chronic corticosteroids for SLE/LN management, the dose must be stable for ≥ 14 days before screening and cannot exceed 20 mg prednisone/day or equivalent at LD start with planned taper to ≤ 5 mg prednisone by the time of LD start with planned taper to ≤ 5 mg prednisone by the time of the first NKX019 infusion.
* Negative SARS-CoV-2 test.

Exclusion Criteria:

* Estimated glomerular filtration rate (eGFR) as calculated by the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation of \< 45 mL/min/1.73 m2 at screening.
* Currently requiring renal dialysis (hemodialysis or peritoneal dialysis) or expected to require dialysis during the study period
* More than 7 g/ day of proteinuria.
* Previous solid organ or hematopoietic cell transplant or planned transplant within study treatment period.
* Congenital or acquired immunodeficiency resulting in severe infection or those receiving chronic immunoglobulin replacement therapy.
* Liver disease or dysfunction, including cirrhosis and/or aspartate aminotransferase, alanine aminotransferase, or bilirubin ≥ 3 times the upper limit of normal.
* Pulmonary comorbidity including chronic obstructive pulmonary disease or asthma requiring daily oral steroids or resting hypoxemia (\< 90% oxygen saturation via pulse oximetry) on room air.
* \>10 pack years of smoking.
* Exacerbation of COPD/asthma requiring systemic steroid therapy within the past 6 months.
* Bone marrow insufficiency unrelated to active SLE (according to Investigator judgment) with white blood cell count \< 1,500/mm3; hemoglobin levels \< 9 g/dL absolute neutrophil count (ANC) \< 1,000/mm3; absolute lymphocyte count \</=500 mm3, or platelet count ≤ 75,000/mm3
* Major cardiac disease, abnormalities, or interventions as defined by, but not limited to:

  1. Uncontrolled angina or unstable life-threatening arrhythmias
  2. History of myocardial infarction within 12 weeks prior to the first dose of NKX019
  3. Any prior coronary artery bypass graft surgery
  4. ≥ Class III New York Heart Association (NYHA) congestive heart failure (CHF), significantly decreased ejection fraction (EF ≤ 45%), or severe cardiac insufficiency
  5. Prolongation of the QT interval corrected for heart rate (QTc) (Fridericia) interval of \> 480 msec
  6. Peripheral artery bypass graft surgery, pulmonary embolism, or other ≥ Grade 2 thrombotic or embolic events within 12 weeks prior to the first dose of NKX019 12. Active CNS SLE . 13. Active bleeding disorders.
* Any overlapping autoimmune condition for which the condition or the treatment of the condition may affect the study assessments or outcomes (e.g. scleroderma with significant pulmonary hypertension; any condition for which additional immunosuppression is indicated). Overlapping conditions for which the condition or treatment is not expected to affect assessments or outcomes (e.g. Sjögren's syndrome) are not excluded.
* Pregnancy, breast feeding or, if of childbearing potential, not using adequate contraceptive precautions or planned oocyte and sperm donation.
* Current/active infection, and any infection requiring systemic antimicrobial therapy within the past 30 days of planned LD.
* History of positive HIV antibody or test positive at screening. Hepatitis B or C positive at screening, active tuberculosis (TB) or latent TB requiring suppressive therapy.
* Major surgery within 28 days prior to the first dose of NKX019 or any surgery from which the subject has not recovered or has ongoing complications.
* Malignancy within 5 years of screening, with the exception of basal and squamous cell carcinomas treated by complete excision. Subjects with cervical dysplasia that is cervical intraepithelial neoplasia but have been treated with conization or loop electrosurgical excision procedure, and have had a normal repeat Papanicolaou test are allowed.
* Prior cellular therapy, including mesenchymal, CAR-T or CAR-NK cells.
* Prior cerebrovascular ischemia/hemorrhage including transient ischemic attack within 90 days prior to the first dose of NKX019.
* Any other acute or chronic medical or psychiatric condition, or known laboratory abnormality that, in the Investigator's opinion is expected to:

  1. Increase the risk associated with study participation or NKX019 administration
  2. Interfere with the informed consent process, compliance with the study requirements, or interpretation of the study results
  3. Make the subject inappropriate for entry into this study
  4. Require concomitant use of any medication that is listed as prohibited while on study
  5. In the opinion of the Investigator, clinically significant drug or alcohol abuse within 2 years prior to screening
* Prior therapies for SLE, including investigational agents, within 4 weeks or 5 half-lives of the drug (whichever is longer) prior to lymphodepletion.
* Currently taking or known need for any of the medications prohibited in the study protocol.
* Known hypersensitivity or contraindications to the study treatment including LD; or other components such as human serum albumin or dimethyl sulfoxide.

Where this trial is running

New York, New York

• Columbia University Irving Medical Center — New York, New York, United States (Recruiting)

Study contacts

• Principal investigator: Anca D Askanase, MD, MPH — Columbia University
• Study coordinator: Anca D Askanase, MD, MPH

Conditions Systemic Lupus Erythematosus; CD19; CAR; Allogeneic; NKX019; Cell Therapy; Lupus Nephritis; Systemic lupus erythematosus