Neoadjuvant chemoradiotherapy versus primary surgery with blood-based tumor cell monitoring for rectal cancer

Impact of Neoadjuvant Chemoradiotherapy Versus Surgery Alone on Circulating Tumor Cells in Patients With Stage II-III Rectal Cancer and Negative Circumferential Resection Margin: a Multicenter Randomized Clinical Trial

Not applicable Interventional University Hospital Ostrava · NCT07194616

We will test whether giving long-course chemoradiotherapy before surgery versus having surgery first changes circulating tumor cells in people with stage II–III rectal cancer who have no circumferential resection margin involvement.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment120 (estimated)
Ages18 Years and up
SexAll
SponsorUniversity Hospital Ostrava Academic / other
Locations4 sites (Olomouc and 3 other locations)
Trial IDNCT07194616 on ClinicalTrials.gov

What this trial studies

This prospective, multicenter randomized trial compares long-course neoadjuvant chemoradiotherapy followed by surgery versus upfront total mesorectal excision in patients with stage II–III rectal cancer without MRI evidence of circumferential resection margin involvement. Participants are randomized to one of the two treatment arms and have serial blood draws at predefined time points to detect and quantify circulating tumor cells (CTCs) as a liquid biopsy biomarker. The primary focus is on the presence and dynamics of CTCs over the treatment course; secondary endpoints include perioperative morbidity and mortality, local recurrence, disease-free survival, and overall survival. The study aims to determine whether CTC monitoring provides prognostic information that could inform the use of neoadjuvant therapy in this patient group.

Who should consider this trial

Good fit: Adults (≥18) with histologically confirmed stage II–III rectal adenocarcinoma within 12 cm of the anal verge, negative circumferential resection margin on staging MRI, ASA physical status I–III, and who can give informed consent.

Not a fit: Patients with metastatic (stage IV) or recurrent rectal cancer, MRI evidence of CRM involvement, other concurrent malignancies, or those unfit for surgery are unlikely to receive benefit from this protocol.

Why it matters

Potential benefit: If successful, the study could help clinicians use a simple blood test to better predict recurrence risk and decide who benefits from preoperative chemoradiotherapy.

How similar studies have performed: Observational studies have linked CTC levels and changes to recurrence risk in colorectal cancer, but randomized data comparing CTC dynamics between neoadjuvant chemoradiotherapy and upfront surgery in CRM-negative rectal cancer are limited.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Age ≥ 18 years
* Histologically confirmed rectal adenocarcinoma within 12 cm from anal verge
* Stage II (cT3-4 N0 M0) or stage III (cT1-4 N1-2, M0)
* Negative circumferential resection margin on staging MRI
* ASA physical status I-III
* Signed informed consent

Exclusion Criteria:

* Tumor infiltration beyond fascia recti propria on MRI
* Metastatic disease (stage IV)
* Recurrent rectal cancer
* Other concurrent malignancies
* Emergency surgery required
* Contraindication to surgery under general anesthesia

Where this trial is running

Olomouc and 3 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Rectal Cancer Patientscirculating tumor cellsliquid biopsyneoadjuvant chemoradiotherapyprognostic biomarkersrectal cancersurgical resection
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.