Infusion of engineered lymphocytes for patients with NY-ESO-1 expressing metastatic cancers

A Phase I/II Dose Escalation, Safety and Efficacy Study of Anti-NY-ESO-1 T Cell Receptor (TCR)-Gene Engineered Lymphocytes Given by Infusion to Patients With NY-ESO-1 -Expressing Metastatic Cancers

This study is testing a new treatment using specially modified immune cells to see if it can help people with certain advanced cancers that have the NY-ESO-1 marker.

Quick facts

• Phase: Phase1; Phase2
• Study type: Interventional
• Enrollment: 3 (estimated)
• Ages: 18 Years to 70 Years
• Sex: All
• Sponsor: Hadassah Medical Organization (Academic / other)
• Drugs / interventions: cyclophosphamide, fludarabine, prednisone
• Locations: 1 site (Jerusalem)
• Trial ID: NCT05296564 on ClinicalTrials.gov

What this trial studies

This clinical trial is a Phase I/II study evaluating the safety and efficacy of HBI 0201-ESO TCR gene-engineered lymphocytes in patients with metastatic cancers that express the NY-ESO-1 antigen. The study consists of two parts: Part A focuses on determining the maximum tolerated dose through a dose escalation design, while Part B aims to assess the clinical response rate at the selected safe dose. A total of up to 63 patients may be enrolled across both phases, with a Data Safety Monitoring Board overseeing the safety of the treatment regimen.

Who should consider this trial

Good fit: Ideal candidates include patients with confirmed metastatic cancers expressing NY-ESO-1 who have previously undergone standard therapies without success.

Not a fit: Patients whose tumors do not express NY-ESO-1 or who have not received prior treatment may not benefit from this study.

Why it matters

Potential benefit: If successful, this treatment could provide a new therapeutic option for patients with difficult-to-treat metastatic cancers.

How similar studies have performed: Other studies utilizing TCR gene-engineered lymphocytes have shown promising results, indicating potential for success in this approach.

Eligibility criteria

Inclusion Criteria:

1. Have histologically or cytologically confirmed diagnosis of neoplasia
2. Measurable (per RECIST v1.1 criteria) metastatic cancer or locally advanced refractory/recurrent malignancy not amenable to curative treatment. Lesions previously irradiated may be considered measurable only if growth has been documented since local treatment completion.
3. The tumor expresses ESO as assessed immunohistochemistry of resected tissue. To this end, archived tumor tissue suitable for analysis must be available or re-biopsy performed on study. Tissue staining must encompass more than 10% of tumor section.
4. Patients must have previously either (1) received at least first-line or second-line standard therapy for metastatic disease, if known to be effective for that disease, and have been either non-responders (progressive disease), intolerable or have recurred or (2) Recurred within 6 months of adjuvant systemic therapy known to be active also in the metastatic setting.
5. Patients with 3 or fewer brain metastases that are less than 1 cm in diameter and asymptomatic are eligible. Lesions that have been treated with stereotactic radiosurgery must be clinically stable for 1 month after treatment for the patient to be eligible. Patients with surgically resected brain metastases are eligible.
6. More than four weeks must have elapsed since any prior systemic therapy at the time the patient receives the preparative regimen, and patients' toxicities must have recovered to a grade 1 or less (except for toxicities such as alopecia or vitiligo).
7. Age ≥ 18 years and ≤ 70 years.
8. Patient is able to understand and willing to sign a written informed consent.
9. Clinical performance status of ECOG 0, 1 or 2.
10. HLA-A\*0201or A\*0206 positive.
11. Patients of both genders must be willing to practice birth control from the time of enrollment on this study and for four months after treatment.
12. Women of child-bearing potential must have a negative pregnancy test.
13. Serology: Seronegative for HIV antibody, hepatitis B antigen, and hepatitis C antibody. If hepatitis C antibody test is positive, then patient must be tested for the presence of antigen by RT-PCR and be HCV RNA negative.
14. Hematology

    * ANC \> 1500/mm3 without the support of filgrastim
    * WBC ≥ 3000/mm3
    * Platelet count ≥ 100,000/mm3
    * Hemoglobin \> 8.0 g/dL. Subjects may be transfused to reach this cut-off.
15. Chemistry

    * Serum ALT/AST ≤ 2.5 x ULN
    * Creatinine clearance ≥40ml/min
    * Total bilirubin ≤ 1.5 mg/dL, except in patients with Gilbert's Syndrome, who must have a total bilirubin \< 3.0 mg/dL.
    * INR \< 1.5

Exclusion Criteria:

1. Women of child-bearing potential who are pregnant or breastfeeding.
2. Any form of primary immunodeficiency (such as Severe Combined Immunodeficiency Disease).
3. Active systemic infections requiring anti-infective treatment, coagulation disorders, or any other active or uncompensated major medical illnesses
4. Concurrent systemic steroid therapy, not including replacement therapy or treatment with prednisone up to 10mg daily or its equivalent. Or any other form of immunosuppressive therapy within 7 days before the first dose of study intervention.
5. History of severe immediate hypersensitivity reaction to cyclophosphamide, fludarabine, or aldesleukin.
6. Subjects with a history of stroke, unstable angina, myocardial infarction, or ventricular arrhythmia requiring medication or mechanical control within 3 months.
7. Subjects unable to maintain normal oxygen saturation level in room air.
8. Subjects who have had a venous thromboembolic event requiring anticoagulation and who meet any of the following criteria:

   * Have been on a stable dose of anticoagulation for \< 1 month (except for acute line insertion induced thrombosis).
   * Have had a Grade 2, 3, or 4 hemorrhage in the last 30 days or are experiencing continued symptoms from their venous thromboembolic event (e.g. continued dyspnea or oxygen requirement).
9. Has a known additional malignancy within the last 3 years. Exceptions include early stage cancers (carcinoma in situ, basal cell carcinoma of the skin, squamous cell carcinoma of the skin, in situ cervical cancer, or in situ breast cancer that has undergone potentially curative therapy).
10. LVEF ≤ 40%
11. Documented FEV1 ≤ 60% predicted tested in patients with:

    * A prolonged history of cigarette smoking (≥ 20 pack-year smoking history, with cessation within the past two years).
    * Symptoms of respiratory dysfunction.
12. Patients who are at the time of study initiation receiving any other investigational agents.
13. Carcinomatosis meningitis or other brain involvement exceeding that allowed above.
14. Has received live vaccine within 30 days before the first dose of study intervention. Examples of live vaccines include, but are not limited to, the following: measles, mumps, rubella, chicken pox, yellow fever, rabies, Bacillus Calmette-Guérin (BCG), and typhoid vaccine. Seasonal influenza vaccines for injection are generally killed virus vaccines and are allowed; however, intranasal influenza vaccines (eg, FluMist®) are live attenuated vaccines and are not allowed.

Where this trial is running

Jerusalem

• Hadassah Medical Organization — Jerusalem, Israel (Recruiting)

Study contacts

• Principal investigator: Michal Lotem, MD — Hadassah Medical Organization
• Study coordinator: Michal Lotem, Prof.

Conditions Sarcoma, Synovial; Sarcoma,Soft Tissue; Melanoma Stage IV; Triple Negative Breast Cancer; Metastatic Cancer; Non Small Cell Lung Cancer; Bladder Urothelial Carcinoma; Neuroblastoma, Metastatic