Fluorescence-enhanced stereotactic biopsy needle for tumor and vessel detection

Fluorescence Enhanced Stereotactic Surgery

Observational Fondazione I.R.C.C.S. Istituto Neurologico Carlo Besta · NCT07286903

This project will test a biopsy needle fitted with 5-ALA fluorescence, Raman spectroscopy, and ultrasound to see if it can identify tumor tissue and blood vessels in adults with high-grade glioma.

Quick facts

Study typeObservational
Enrollment20 (estimated)
Ages18 Years and up
SexAll
SponsorFondazione I.R.C.C.S. Istituto Neurologico Carlo Besta Academic / other
Locations1 site (Milan)
Trial IDNCT07286903 on ClinicalTrials.gov

What this trial studies

This prospective, multicenter observational project develops a fiber-optic probe integrated into a standard Nashold stereotactic biopsy needle. The device combines 5-ALA–driven fluorescence, Raman spectroscopy, and ultrasound imaging to provide real-time signals intended to distinguish tumor tissue from normal brain and to identify vascular structures. Ex-vivo testing will be performed on resected intra-axial high-grade glioma samples, with probe readings correlated to histopathology across phased development. The program moves from prototyping through phantom and tissue testing to optimization and final validation using approximately 20 tumor samples.

Who should consider this trial

Good fit: Adults (≥18 years) undergoing surgical resection for a suspected high-grade glioma who will receive 5-ALA and can provide informed consent are appropriate candidates.

Not a fit: Patients who are not surgical candidates, cannot receive 5-ALA, or cannot provide informed consent are unlikely to benefit from this ex-vivo device testing.

Why it matters

Potential benefit: If successful, this technology could reduce non-diagnostic biopsies and lower the risk of hemorrhage by helping clinicians better identify tumor margins and nearby blood vessels.

How similar studies have performed: 5-ALA fluorescence is an established aid in glioma surgery, but combining fluorescence with Raman spectroscopy and ultrasound inside a biopsy needle is novel and has only limited prior validation in patient-derived tissues.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Patient aged 18 years or older
* Patients able to provide the informed consentPresumptive diagnosis of high grade glioma
* Patient whose surgery require the use of 5-ALA as standard of treatment

Exclusion Criteria:

* Patients who are not suitable for surgery
* Patients unable to provide informed consent due to cognitive impairment or other reasons

Where this trial is running

Milan

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions High Grade GliomaStereotactic BiopsyFluorescence Guided Surgery5-ALARaman SpectroscopyGliomaFiber OptictsNashold Needle
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.