Fasting for 84 Hours: How It Affects Fat Burning and Muscle Basics in Young Adults
Official title: The Effect of Fasting on Peak Fat Oxidation, Mitochondrial Coupling and Extracellular Matrix in Healthy Men and Lean and Obese Men and Women
Run by University of Copenhagen · NCT07692646 · Data updated 2026-11-12
This study tests whether 84 hours of fasting changes peak fat oxidation, how efficiently mitochondria couple their energy, and the extracellular matrix in four groups of young people who are normal weight or obese.
Quick facts
| Study type | Interventional |
|---|---|
| Ages | 18 Years to 40 Years |
| Sex | All |
| Enrollment | 40 (estimated) |
| Sponsor | University of Copenhagen Academic / other |
| Locations | 1 site (Copenhagen) |
| Status | Recruiting |
| Started | June 2026 |
| Main results expected | August 2027 |
| Study ends | December 2033 |
| Registry record updated | July 9, 2026 |
| Trial ID | NCT07692646 on ClinicalTrials.gov |
Is this trial a fit for you?
✓Likely a good fit
Ideal candidates are healthy men and women aged 18-40 with BMI 18-25 or 30-40 who can comply with dietary records and fasting procedures.
✕Probably not a fit
Participants who do not experience changes in fat metabolism or who have adverse reactions to fasting may not see direct benefits.
A plain-English reading of the study's rules. Only the study team can confirm whether you qualify.
What this trial studies
Four groups of young adults (10 per group) will fast for 84 hours and have measurements taken before and after. The study uses DXA for body composition, indirect calorimetry for resting energy expenditure, blood tests, and tissue biopsies from muscle and abdominal fat. Participants follow a measured dietary and activity plan before testing and consent is obtained after information is provided. The goal is to see if fasting alters fat metabolism and tissue properties differently by weight status and sex.
Why it matters
Potential benefit: If successful, the findings could improve understanding of how prolonged fasting affects fat use and muscle biology in young adults with varying BMI.
How similar studies have gone: Prior research has explored fasting effects on metabolism, but this study is unique in combining fat oxidation, mitochondrial coupling, and extracellular matrix analysis across weight groups.
Who can join: the full rules
Show full inclusion / exclusion criteria
Inclusion Criteria: * Healthy normal weight men aged \[18,40\] and BMI \[18,25\] * Healthy normal weight women \[18,40\] and BMI \[18,25\] * Healthy obese men \[18,40\] and BMI \[30,40\] * Healthy obese women \[18,40\] and BMI \[30,40\] Exclusion Criteria: * Present or former cardiovascular disease and chronical inflammatory disease * Consumption of medications and supplements that influence substrate use at rest and/or during exercise * Pregnancy
Where it's running
-
Department of Biomedical Sciences
Copenhagen, Denmark Recruiting
Study contacts
- Study coordinator: Jørn W Helge, PhD
- Email: jhelge@sund.ku.dk
- Phone: +4528757506
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How to take part
- Review the eligibility criteria above with your treating physician.
- Check the official listing on ClinicalTrials.gov for the latest contact details and recruitment status.
- Contact the study coordinator or principal investigator to ask for pre-screening. Pre-screening is free and never obligates you to enroll.