Continuous versus standard intermittent ampicillin plus ceftriaxone for Enterococcus faecalis endocarditis

Efficacy and Safety of Ampicillin and Ceftriaxone Continuous Infusion Versus Standard Therapy for the Treatment of Enterococcus Faecalis Infective Endocarditis (DOβLEI Study)

Phase 4 Interventional Fundación Pública Andaluza para la gestión de la Investigación en Sevilla · NCT06423898

This study will see if giving ampicillin plus ceftriaxone as a continuous infusion works as well and is as safe as the usual intermittent dosing for adults with Enterococcus faecalis infective endocarditis.

Quick facts

PhasePhase 4
Study typeInterventional
Enrollment284 (estimated)
Ages18 Years and up
SexAll
SponsorFundación Pública Andaluza para la gestión de la Investigación en Sevilla Academic / other
Locations18 sites (Alava and 17 other locations)
Trial IDNCT06423898 on ClinicalTrials.gov

What this trial studies

This is a phase IV, open-label, randomized multicenter non-inferiority trial comparing continuous infusion of ampicillin plus ceftriaxone with the standard intermittent regimen for Enterococcus faecalis infective endocarditis. Adult patients with a possible or definite diagnosis are randomized to continuous infusion or standard care, with most treatment delivered in settings approximating routine outpatient care. Key outcomes include treatment success, mortality, serious adverse events, total antibiotic days, and days of hospitalization, with treatment lasting around 42 days and final follow-up at 365 days after treatment. The protocol is pragmatic, aiming to keep study visits similar to normal clinical follow-up to reflect real-world practice.

Who should consider this trial

Good fit: Adults with a possible or definite diagnosis of Enterococcus faecalis infective endocarditis who can give informed consent and do not have allergy to penicillins/cephalosporins, pregnancy/lactation, or polymicrobial infection.

Not a fit: Patients with penicillin or cephalosporin allergy, pregnancy or lactation, polymicrobial infections including organisms other than E. faecalis, or clinical situations requiring prolonged inpatient care are unlikely to benefit from this approach.

Why it matters

Potential benefit: If successful, the continuous-infusion approach could shorten hospital stays, lower hospital-related complications, and allow more treatment to be given safely as outpatient care.

How similar studies have performed: Smaller observational studies and outpatient infusion programs have suggested continuous beta-lactam infusions can be effective, but high-quality randomized non-inferiority data specific to E. faecalis endocarditis remain limited.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Adult patients
* Possible or definitive diagnosis of infective endocarditis due to Enterococcus faecalis
* Signed informed consent of patients

Exclusion Criteria:

* Allergy to penicillins or cephalosporins
* Pregnancy and lactation
* Polymicrobial infection including microorganisms different to E. faecalis

Where this trial is running

Alava and 17 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Endocarditis, InfectiveEnterococcus faecalisAntibioticsOutpatient Infusion TherapiesOutpatient Care
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.