Comparing ICSI versus conventional IVF using frozen third‑party donor sperm

Prospective Randomized Sibling-Oocyte Trial Comparing Fertilization and Embryo Development Outcomes Between Intracytoplasmic Sperm Injection (ICSI) and Conventional IVF Using Frozen Donor Sperm

Not applicable Interventional Shady Grove Fertility Reproductive Science Center · NCT07175571

This trial tests whether injecting sperm into eggs (ICSI) or mixing eggs with frozen donor sperm (conventional IVF) makes more and better embryos for people using frozen donor sperm without male‑factor infertility.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment95 (estimated)
SexFemale
SponsorShady Grove Fertility Reproductive Science Center Academic / other
Locations1 site (Rockville, Maryland)
Trial IDNCT07175571 on ClinicalTrials.gov

What this trial studies

This is a prospective, randomized sibling‑oocyte trial in which each participant's retrieved eggs are split and randomly assigned to either ICSI or conventional IVF using cryopreserved donor sperm. The within‑patient design reduces variability from individual egg and ovarian differences and allows direct comparison of fertilization techniques. Primary outcomes include number of usable blastocysts and incidence of total fertilization failure, with secondary outcomes of embryo quality and early pregnancy rates. The trial is conducted at a single center with participants required to have normal ovarian reserve and more than four oocytes retrieved.

Who should consider this trial

Good fit: People undergoing IVF with frozen donor sperm who have normal ovarian reserve, no male‑factor infertility, and are expected to produce more than four oocytes are ideal candidates.

Not a fit: Patients with male‑factor infertility, those using partner sperm, people with low ovarian reserve, or those unlikely to produce at least four oocytes are unlikely to benefit from this trial.

Why it matters

Potential benefit: If successful, the trial could identify which fertilization method yields more usable embryos from frozen donor sperm, helping clinicians choose the simpler or safer approach and potentially avoiding unnecessary ICSI.

How similar studies have performed: Retrospective studies and meta‑analyses suggest ICSI may reduce total fertilization failure but do not consistently show improved blastulation or pregnancy in non‑male factor cycles, and randomized sibling‑oocyte data remain limited.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Female patients undergoing IVF with frozen donor sperm at Shady Grove Fertility Center
* Normal ovarian reserve (AMH \> 1 ng/mL, AFC \> 10)
* Absence of male factor infertility
* Post-wash parameters: \>50% motility, \>5mil concentration
* \> 4 oocytes retrieved at time of transvaginal oocyte retrieval

Exclusion Criteria:

* Donor Sperm with significant male factor infertility (e.g., abnormal sperm concentration, motility, or morphology)
* Any medical condition contraindicating ART

Where this trial is running

Rockville, Maryland

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Infertility, FemaleReproductive Techniques, AssistedFertilization in VitroIntracytoplasmic Sperm InjectionConventional IVFFrozen Donor SpermSibling-Oocyte DesignNon-Male Factor Infertility
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.