Cell-free tsRNA signature to detect early liver cancer

A Cell-Free tsRNA-Based Liquid Biopsy Signature for Early Detection of Hepatocellular Carcinoma

Observational City of Hope Medical Center · NCT07265271

This project will test whether a blood test that measures cell-free tsRNAs can detect early-stage hepatocellular carcinoma using samples from people with confirmed HCC.

Quick facts

Study typeObservational
Enrollment600 (estimated)
Ages18 Years and up
SexAll
SponsorCity of Hope Medical Center Academic / other
Drugs / interventionschemotherapy
Locations1 site (Duarte, California)
Trial IDNCT07265271 on ClinicalTrials.gov

What this trial studies

This observational study collects pre-treatment blood samples from patients with histologically confirmed hepatocellular carcinoma at City of Hope to develop a liquid biopsy based on cell-free tsRNAs. Investigators will use small RNA sequencing to identify candidate cf-tsRNA markers and then validate selected signatures with RT-qPCR. The work aims to produce a sensitive, noninvasive assay to improve detection of early-stage HCC where current markers like AFP have limited sensitivity. Findings will be compared to clinical staging and standard diagnostic work-ups to see how well the cf-tsRNA signature distinguishes early disease.

Who should consider this trial

Good fit: Adults with histologically confirmed hepatocellular carcinoma who have not yet received curative-intent treatment and can provide a pre-treatment blood sample and informed consent.

Not a fit: People without HCC, those unable to give informed consent, or patients who have already started curative-intent therapy prior to blood collection are unlikely to benefit from this protocol.

Why it matters

Potential benefit: If successful, this could enable earlier, noninvasive detection of HCC so more patients can receive curative treatment and have better survival chances.

How similar studies have performed: Related liquid-biopsy approaches using cfDNA and circulating small RNAs (like miRNAs) have shown promise for liver cancer, but cf-tsRNA-based diagnostics are relatively novel and supported mainly by early-stage data.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* A histologically confirmed diagnosis of hepatocellular carcinoma.
* Received standard diagnostic and staging procedures as per local guidelines
* Availability of at least one blood-derived sample, drawn before receiving any curative-intent treatment

Exclusion Criteria:

• Lack of or inability to provide informed consent

Where this trial is running

Duarte, California

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Hepatocellular CarcinomaHCCLiver cancertsRNALiquid BiopsyEarly Detection
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.