Breathing muscle training plus dietary nitrate to improve exercise at simulated moderate altitude

Impact of Respiratory Muscle Training and Nitrate Therapy on Exercise Tolerance in Simulated Altitude

Not applicable Interventional Embry-Riddle Aeronautical University · NCT07216755

This project tests whether breathing muscle training and beetroot-derived nitrate help healthy, active adults exercise better under simulated moderate-altitude (low-oxygen) conditions.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment80 (estimated)
Ages18 Years to 40 Years
SexAll
SponsorEmbry-Riddle Aeronautical University Academic / other
Locations1 site (Daytona Beach, Florida)
Trial IDNCT07216755 on ClinicalTrials.gov

What this trial studies

Healthy, recreationally active adults are randomly assigned to one of four groups combining respiratory muscle training or a sham device with beetroot nitrate supplement or a placebo beverage. Interventions include a targeted respiratory muscle training program and dietary nitrate (beetroot juice concentrate) given before exercise, with controls for sham training and placebo beverage. Participants perform cycle ergometer tests to volitional fatigue under simulated reduced-oxygen conditions while investigators measure exercise tolerance, oxygen delivery, and physiological strain. The design compares effects of each intervention alone and in combination to see whether they independently or synergistically improve performance in hypoxia.

Who should consider this trial

Good fit: Healthy, recreationally active adults aged 18–40 who are non-smokers, free of known cardiovascular, pulmonary, metabolic, or neuromuscular disease, and able to perform maximal cycle ergometer exercise are ideal candidates.

Not a fit: People with heart, lung, metabolic, or neuromuscular disease, recent smokers or vapers, those taking medications or supplements that affect cardiovascular or respiratory function, or those unable to perform maximal cycling are unlikely to benefit and are excluded.

Why it matters

Potential benefit: If successful, these low-cost, non-drug approaches could help healthy people and operational personnel maintain endurance and reduce fatigue in low-oxygen environments.

How similar studies have performed: Prior studies show that respiratory muscle training and dietary nitrate can each improve exercise performance or oxygen delivery in hypoxic conditions, but combining the two is less well studied and remains relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Healthy adults between 18 and 40 years old.

Recreationally active (performing ≥3 hours of structured physical activity per week).

Non-smokers for at least the past 6 months.

Free from any known cardiovascular, pulmonary, metabolic, or neuromuscular disease as determined by health screening questionnaire and medical history.

Able to perform cycle ergometer exercise to volitional fatigue.

Willing to abstain from high-nitrate foods and supplements (e.g., beets, spinach, arugula) for 48 hours prior to each testing session.

Able and willing to provide written informed consent and comply with all study procedures.

Exclusion Criteria:

* Diagnosis of or history of heart disease, hypertension, diabetes, or chronic respiratory disorders (e.g., asthma, COPD).

Current use of medications or supplements known to affect cardiovascular, metabolic, or respiratory function (e.g., beta-blockers, nitrates, stimulants).

Smoking, vaping, or tobacco use within the past 6 months.

Known allergy or intolerance to beetroot products or nitrates.

Participation in another interventional research study within the past 30 days.

Pregnant or breastfeeding women.

Any orthopedic or musculoskeletal limitation preventing safe exercise testing.

Failure to meet inclusion criteria or inability to complete familiarization and baseline testing.

Where this trial is running

Daytona Beach, Florida

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Healthy AdultsMechanical Ventilation DependenceHypoxiaExercise TrainingExercise IntoleranceDietary SupplementNitrate SupplementationInspiratory Muscle Training
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.