Assessing biochemical profiles in migraine patients

Unraveling the Spectrum of Migraine Resistant to Treatments: Searching for Novel Biological PHEnotypes and theRApeutic Approaches (SPHERA Project)

Observational IRCCS National Neurological Institute "C. Mondino" Foundation · NCT06549270

This study is testing if certain biological markers can help explain why some people with chronic and episodic migraines respond better to a new preventive treatment than others.

Quick facts

Study typeObservational
Enrollment100 (estimated)
Ages18 Years to 75 Years
SexAll
SponsorIRCCS National Neurological Institute "C. Mondino" Foundation Academic / other
Locations1 site (Pavia)
Trial IDNCT06549270 on ClinicalTrials.gov

What this trial studies

This observational study aims to evaluate the dysfunction of the endocannabinoid system in patients with chronic and episodic migraines. Participants will undergo preventive therapy with monoclonal antibodies targeting calcitonin gene-related peptide and will be assessed for neurochemical biomarkers, including neuropeptides, cytokines, and microRNAs. The primary goal is to compare patients who experience significant reductions in migraine days after treatment with those who do not, identifying potential biomarkers that could explain treatment resistance. This research is part of the SPHERA project and seeks to deepen our understanding of migraine pathophysiology.

Who should consider this trial

Good fit: Ideal candidates include adults aged 18 to 75 with a diagnosis of episodic or chronic migraine who have not previously received CGRP-targeting treatments.

Not a fit: Patients with other primary or secondary headache disorders, significant psychiatric or neurological conditions, or those currently undergoing changes in preventive treatment may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to better-targeted treatments for migraine patients who do not respond to current therapies.

How similar studies have performed: While there is evidence of altered endocannabinoid systems in migraine patients, this specific approach to deep phenotyping and biomarker identification is relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* male and female patients aged 18 to 75 years
* diagnosis of episodic migraine or chronic migraine according to ICHD-3 criteria
* for episodic migraine: 8-14 monthly migraine days in the previous 3 months
* diagnosis of resistant migraine defined by: i) having failed at least 3 classes of migraine preventatives and ii) suffering from at least 8 debilitating monthly headache days for at least 3 consecutive months
* patients naive to CGRP targeting treatments

Exclusion Criteria:

* history of major psychiatric or other neurological conditions
* diagnosis of other primary or secondary headache disorders (only sporadic tension-type headache is allowed if the patients can clearly differentiate between the 2 types of headaches)
* changes in ongoing preventive treatment (if any) in the previous 3 months
* clinically significant medical conditions
* chronic pain conditions
* alcohol and/or drug abuse
* pregnancy or lactation

Where this trial is running

Pavia

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Migraine DisordersChronic MigraineEpisodic Migrainemigraineanti CGRP mABscalcitonin gene related peptideendocannabinoid system
Last reviewed 2026-06-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.