Proteins that interact with different WT1 versions
Identification of isoform-specific WT1 interaction partners
This project looks for proteins that bind to different forms of the WT1 gene to help explain Wilms tumor, kidney disease, and related disorders.
Quick facts
| Grant type | R03 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Research Inst Nationwide Children's Hosp NIH-funded |
| Lab location | 1 site (Columbus, United States) |
| Project ID | NIH-11240336 on NIH RePORTER |
What this research studies
Researchers will compare common WT1 isoforms (including +KTS and -KTS) and disease-linked WT1 mutants to see which proteins they interact with. They will use tagging and proximity-labeling techniques to mark nearby proteins, capture those partners with affinity methods, and identify them by quantitative mass spectrometry. By comparing interaction partners across isoforms and mutants, the team hopes to clarify how different WT1 forms perform distinct roles in kidney cells and tumors. The work is lab-based using molecular and proteomic tools rather than testing treatments in people.
Who could benefit from this research
Good fit: People with WT1-related conditions—such as Wilms tumor, hereditary nephropathy linked to WT1 mutations, or disorders of sex differentiation tied to WT1—would be the most relevant candidates for sample donation or future clinical follow-up.
Not a fit: Patients with unrelated illnesses or those expecting immediate therapeutic benefit should not expect direct clinical help from this laboratory-focused project.
Why it matters
Potential benefit: If successful, the findings could reveal biomarkers or molecular targets that eventually lead to better diagnosis or therapies for Wilms tumor and WT1-related kidney disease.
How similar studies have performed: Proteomic and proximity-labeling approaches have successfully mapped partners for other transcription factors, though applying these methods specifically to WT1 isoforms is relatively new.
Where this research is happening
Columbus, United States
- Research Inst Nationwide Children's Hosp — Columbus, United States (Active)
Researchers
- Principal investigator: Mann, Nina — Research Inst Nationwide Children's Hosp
- Study coordinator: Mann, Nina
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.