Multiparametric fluorescence imaging to link metabolism with cellular changes
Official title: Developing multiparametric fluorescence microscopy to connect metabolism with cellular manipulations
Funded at Georgetown University · NIH-11382191 · Data updated 2026-11-12
A grant to develop advanced fluorescence imaging methods that can show how metabolism, protein movement, and membrane properties relate to each other in living cells.
Quick facts
| Grant type | NIH-funded research |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Georgetown University NIH-funded |
| Lab location | 1 site (Washington, United States) |
| Project start | September 1, 2024 |
| Project end | July 31, 2029 |
| Funding year | 2026 |
| Award (this year) | $390,000 |
| NIH project number | 5R35GM154815-03 |
| Project ID | NIH-11382191 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates are individuals whose cells can be studied with advanced fluorescence microscopy (e.g., researchers using human-derived cell samples or model systems) in a lab setting.
✕Less likely to help
Patients whose condition cannot be studied with the proposed imaging methods or where samples are not available may not benefit directly.
What this research studies
This project aims to build a seamless imaging workflow that combines several imaging approaches to measure metabolism, protein diffusion, and membrane fluidity at the same high spatial resolution. By using phasor-based FLIM, hyperspectral phasors, and phasor-FCS, researchers will compare how changes in one property affect others within the same cells. The work focuses on correlating physical and metabolic signals to uncover causal relationships in cellular systems. The goal is to enable researchers to observe multiple cellular properties simultaneously, at native image resolution, using confocal and multiphoton microscopy.
Why it matters
Potential benefit: If successful, patients could benefit from more precise biomedical imaging tools that reveal how cellular metabolism and physical properties influence disease processes.
How similar studies have gone: Previous work shows that advanced fluorescence imaging can reveal relationships between cellular properties, but this project aims for an integrated, pixel-by-pixel correlation across modalities.
Where this research is happening
-
Georgetown University
Washington, United States Active
Researchers
- Principal investigator: Ranjit, Suman — Georgetown University
- Study coordinator: Ranjit, Suman
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.