Improving RNA sequencing accuracy using a novel method
Official title: Twice reading of RNA by direct nanopore sequencing
Funded at Thomas Jefferson University · NIH-11081654 · Data updated 2026-11-12
This study is testing a new way to read RNA more accurately using a special technique that could help us better understand how genes work, which might lead to better tests and treatments for patients.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Thomas Jefferson University NIH-funded |
| Lab location | 1 site (Philadelphia, United States) |
| Project start | May 1, 2024 |
| Project end | February 29, 2028 |
| Funding year | 2025 |
| Award (this year) | $843,060 |
| NIH project number | 5R01HG013302-02 |
| Project ID | NIH-11081654 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates for this research are individuals with conditions related to gene expression and RNA modifications, such as certain cancers or genetic disorders.
✕Less likely to help
Patients with conditions unrelated to RNA modifications or those who do not have any genetic disorders may not benefit from this research.
What this research studies
This research focuses on enhancing the accuracy of RNA sequencing by employing a technique called 'twice reading' using a specialized enzyme from the poliovirus. By copying RNA modifications twice, the study aims to provide a more precise mapping of these modifications, which are crucial for understanding gene expression and their roles in health and disease. The approach utilizes advanced nanopore sequencing technology to capture and quantify RNA modifications that are often missed in traditional methods. Patients may benefit from improved diagnostic tools and treatments based on a better understanding of RNA modifications.
Why it matters
Potential benefit: If successful, this research could lead to more accurate diagnostics and targeted therapies for diseases influenced by RNA modifications.
How similar studies have gone: Previous research has shown promise in improving RNA sequencing techniques, but this specific approach of 'twice reading' is relatively novel and untested.
Where this research is happening
-
Thomas Jefferson University
Philadelphia, United States Active
Researchers
- Principal investigator: Hou, Ya-Ming — Thomas Jefferson University
- Study coordinator: Hou, Ya-Ming
Related research
Other NIH-funded projects on the same topics.
-
Understanding CAG repeat instability in Huntington’s disease
A Huntington’s disease study explores why the CAG repeat in the HTT gene becomes unstable and leads to earlier disease, aiming to uncover factors that influence when and how the disease starts.
-
Noninvasive portal pressure estimation using ultrasound-based subharmonic signals
A noninvasive ultrasound method to estimate portal vein pressure in adults with cirrhosis to help manage varices and ascites.
-
Twice-reading RNA sequencing to better map RNA modifications
Researchers are using a novel method to read RNA twice to more accurately map chemical modifications in human transcripts, which could help us understand health and disease.
-
Improving HIV Prevention for Women in ObGyn Care
Aimed at helping women in obstetric/gynecologic care reduce their risk of HIV by making PrEP and risk–screening more accessible and acceptable.
-
Immunostimulatory short non-coding RNAs and lupus
A study in people with lupus looks at tiny RNA pieces that may spark inflammation, to understand if they drive disease activity.
-
Global Network for Women’s and Children’s Health: New Cycle Leadership and Collaboration
A long-standing collaboration between TJU and JNMC is expanding to lead and coordinate efforts to improve the health of mothers and young children in low-resource settings.
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.