Creating advanced gene editing tools for zebrafish
Official title: Developing robust genome editing tools for generating floxed alleles and editing amino acid change in zebrafish
Funded at University of Wisconsin-Madison · NIH-11129224 · Data updated 2026-11-12
This study is working on better ways to edit genes in zebrafish, which are great for learning about how living things grow and heal, so scientists can more accurately explore how specific genes work in different cells and stages of development.
Quick facts
| Grant type | R21 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | University of Wisconsin-Madison NIH-funded |
| Lab location | 1 site (Madison, United States) |
| Project start | May 1, 2025 |
| Project end | April 30, 2027 |
| Funding year | 2025 |
| Award (this year) | $194,375 |
| NIH project number | 1R21OD037634-01A1 |
| Project ID | NIH-11129224 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates for benefiting from this research include individuals with genetic disorders that could be modeled in zebrafish or those interested in developmental biology.
✕Less likely to help
Patients with conditions that do not have a relevant zebrafish model or those not interested in genetic research may not benefit from this research.
What this research studies
This research focuses on improving gene editing techniques in zebrafish, a valuable model for studying development and regeneration. The team aims to develop a reliable method for creating conditional loss-of-function alleles, which will allow researchers to study gene functions in specific cell types and at different developmental stages. By using a synthetic approach to integrate loxP sites into target genes, the researchers hope to enhance the precision of genetic modifications. This work could significantly advance our understanding of gene functions and their implications in various biological processes.
Why it matters
Potential benefit: If successful, this research could lead to more effective gene editing tools that enhance our understanding of genetic diseases and developmental biology.
How similar studies have gone: Other research has shown success with similar gene editing approaches in various model organisms, indicating a promising potential for this methodology in zebrafish.
Where this research is happening
-
University of Wisconsin-Madison
Madison, United States Active
Researchers
- Principal investigator: Kang, Junsu — University of Wisconsin-Madison
- Study coordinator: Kang, Junsu
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.