Using DNase I to treat sepsis in ICU patients

Intravenous DNase I for the Treatment of Sepsis: A Phase I Safety and Feasibility Study in ICU Patients

Phase 1 Interventional McMaster University · NCT05453695

This study tests if a new treatment called DNase I can help improve recovery for ICU patients with sepsis by breaking down harmful substances in their bodies.

Quick facts

PhasePhase 1
Study typeInterventional
Enrollment36 (estimated)
Ages18 Years to 100 Years
SexAll
SponsorMcMaster University Academic / other
Locations1 site (Hamilton, Ontario)
Trial IDNCT05453695 on ClinicalTrials.gov

What this trial studies

This Phase I trial investigates the safety and feasibility of intravenous DNase I in patients with sepsis admitted to the ICU. The study aims to determine the maximum tolerated dose of DNase I while evaluating its effects on organ dysfunction, ICU length of stay, and mortality rates. By dismantling neutrophil extracellular traps (NETs) that contribute to organ damage, DNase I may improve patient outcomes in critically ill septic patients. The trial will also collect samples for future research on coagulation and immune function in sepsis.

Who should consider this trial

Good fit: Ideal candidates are adults aged 18 and older who have been admitted to the ICU within the last 48 hours with suspected or proven infections and a SOFA score of 2 or higher.

Not a fit: Patients with other forms of shock or significant bleeding risks may not benefit from this treatment.

Why it matters

Potential benefit: If successful, this treatment could significantly improve recovery and survival rates for patients suffering from sepsis.

How similar studies have performed: While this approach is novel, previous animal studies have shown promise for DNase I in treating sepsis.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

1. Age of ≥18 years
2. Admitted to the ICU in the last 48 hours
3. Suspected or proven infection as the admitting diagnosis
4. A sequential (sepsis) organ function assessment (SOFA) score of ≥2 above baseline
5. Expected to remain in the ICU for ≥ 72 hours

Exclusion Criteria:

1. No consent/inability to obtain consent from a substitute decision-maker
2. Have other forms of clinically apparent shock, including cardiogenic, obstructive (massive pulmonary embolism, cardiac tamponade, tension pneumothorax), hemorrhagic, neurogenic, or anaphylactic shock
3. Have a significant risk of bleeding as evidenced by one of the following:

   * Surgery requiring general or spinal anesthesia within 24 hours before enrolment
   * The potential need for surgery in the next 24 hours
   * Evidence of active bleeding
   * A history of severe head trauma requiring hospitalization
   * Intracranial surgery, or stroke within three months before the study
   * Any history of intracerebral arteriovenous malformation, cerebral aneurysm, or mass lesions of the central nervous system
   * A history of congenital bleeding diatheses
   * Gastrointestinal bleeding within five weeks before the study unless corrective surgery had been performed
   * Trauma is considered to increase the risk of bleeding
   * Presence of an epidural catheter
   * Need for therapeutic anticoagulation
4. Receiving DNase I by inhalation
5. Terminal illness with a life expectancy of fewer than three months
6. Pregnant and/or breastfeeding

Where this trial is running

Hamilton, Ontario

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions SepsisCritical Illness
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.