Testing embryo biopsies to improve IVF success rates

Comprehensive Chromosomal Testing of Trophectoderm Biopsies of Blastocysts to Improve Live Birth Rates After in Vitro Fertilization: a Prospective Randomized Trial

NA · Assistance Publique - Hôpitaux de Paris · NCT04758819

This study is testing if a new way of checking embryos can help women aged 35 to 41 have more successful pregnancies through IVF.

Quick facts

PhaseNA
Study typeInterventional
Enrollment700 (estimated)
Ages35 Years to 41 Years
SexAll
SponsorAssistance Publique - Hôpitaux de Paris (other)
Locations10 sites (Bondy and 9 other locations)
Trial IDNCT04758819 on ClinicalTrials.gov

What this trial studies

This clinical trial investigates the effectiveness of Comprehensive Chromosomal Testing of Trophectoderm biopsies from blastocysts in improving live birth rates for women aged 35 to 41 undergoing in vitro fertilization (IVF). The study aims to compare outcomes between traditional embryo selection methods and those utilizing next-generation sequencing to identify euploid embryos. By focusing on older couples facing infertility, the trial seeks to provide a more reliable approach to embryo selection, potentially reducing miscarriage rates and increasing successful pregnancies. Participants will undergo a randomized controlled trial to assess the impact of this testing on their IVF outcomes.

Who should consider this trial

Good fit: Ideal candidates are women aged 35 to 41 with infertility issues who are eligible for assisted reproductive technologies.

Not a fit: Patients with recurrent implantation failures or significant uterine abnormalities may not benefit from this study.

Why it matters

Potential benefit: If successful, this approach could significantly enhance the chances of live births for older women undergoing IVF.

How similar studies have performed: Previous studies have suggested the potential benefits of chromosomal testing in IVF, but this specific randomized controlled trial approach is novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria

Women :

* Age ≥35 to ≤ 41 years old (according to date of birth at time of informed consent) who are eligible for ovarian stimulation and ART treatment, including intracytoplasmic sperm injection (ICSI)
* BMI=18-35 kg/m2 inclusive
* No intrauterine and/or endometrial abnormalities that would interfere with implantation or pregnancy (for instance polyp, fibroid, ...)

Inclusion Criteria Men:

* Use of ejaculated motile sperm (donated and/or cryopreserved sperm is allowed)
* Age ≤ 50 years old

Inclusion Criteria Couples:

* Primary or secondary infertility
* Dated and signed inform consent
* Affiliated to National Insurance
* French speaking, able to understand the study

Criteria after randomization

Couple having at least one blastocyst with morphological score on Day 5/6 of in vitro embryo development (blastocoel expansion ≥3 and inner cell mass graded A, B or C and trophectoderm graded A or B

Exclusion Criteria:

Women:

* Recurrent implantation failure (previous transfer of least 5 good grade blastocysts in at least 3 fresh or frozen cycles)
* Personal history of recurrent miscarriages (more than two miscarriages)
* Altered ovarian reserve: Identified risk of poor ovarian response (history of oocyte puncture with less than 3 oocytes) or AMH\<1.1 ng/mL and AFC\<5)
* Presence of non isolated uni- or bilateral hydrosalpinx
* History or presence of ovarian, uterine or mammary cancer
* Contraindication to being pregnant and/or carrying a pregnancy to term
* Women with uterine polyps diagnosed during COS
* Known allergy or hypersensitivity to human gonadotropin preparations or to compounds that are structurally similar to any of the other medications administered during the trial
* Substance abuse that would interfere with trial conduct, as determined by the investigator
* Pregnant patient, nursing patient

Men:

- Use of testicular or epididymal sperm

Couples:

* Known infection with human immunodeficiency virus, active hepatitis B or C virus in the female or male partner
* Scheduled for an embryo transfer on day 2 or 3
* Embryo freezing refusal
* Scheduled for a fresh embryo transfer
* Scheduled with an egg donation
* Scheduled with autologous oocytes thawing
* Scheduled for a preimplantation genetic diagnosis
* Participation in another ART clinical trial within the past 30 days
* Participation with another interventional study involving human subjects

Exlusion criteria to check on randomization day :

- Women with less than 3 follicles ≥ 14 mm on the triggering day or the day before the triggering

Where this trial is running

Bondy and 9 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.

View on ClinicalTrials.gov →

Conditions: Infertility, embryo aneuploidy, Intracytoplasmic Sperm Injection, in vitro fertilization, Next-Generation Sequencing, randomized controlled trial

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.