Taking calcium and iron-containing multivitamin supplements together during pregnancy

Co-administration of Calcium and Multiple Micronutrient Supplements for Maternal and Newborn Hemoglobin and Iron Status: A Randomized Non-inferiority Trial in Burkina Faso and Pakistan

Phase 3 Interventional Johns Hopkins Bloomberg School of Public Health · NCT06568315

This trial will test whether pregnant women can take daily calcium at the same time as iron-containing multiple micronutrients without harming their or their newborns' hemoglobin and iron levels.

Quick facts

PhasePhase 3
Study typeInterventional
Enrollment3200 (estimated)
Ages15 Years and up
SexFemale
SponsorJohns Hopkins Bloomberg School of Public Health Academic / other
Locations2 sites (Ouagadougou and 1 other locations)
Trial IDNCT06568315 on ClinicalTrials.gov

What this trial studies

Pregnant women 6 to <20 weeks gestation are randomly assigned to take a multiple micronutrient supplement and calcium either together each morning or separated (micronutrient in the morning and calcium in the evening). Participants attend monthly antenatal visits with blood samples collected in early, mid, and late pregnancy and cord or infant blood sampled at birth to measure hemoglobin and iron status. The trial is conducted at study clinics in Burkina Faso and Pakistan and requires participants to receive antenatal care at the study site and stop routine iron-folic acid supplements while enrolled. The aim is to determine whether co-administration affects iron status and whether a simpler dosing schedule could improve adherence and inform future supplement formulations.

Who should consider this trial

Good fit: Pregnant women in the study sites (Burkina Faso or Pakistan) between 6 and <20 weeks gestation with hemoglobin ≥70 g/L who are willing to receive all antenatal care at a study clinic and replace routine iron-folic acid with the study supplements.

Not a fit: Women with severe anemia (hemoglobin <70 g/L), those outside the 6–<20 week gestation window, or those unwilling to attend the study clinic or stop routine iron-folic acid may not benefit from or be eligible for this intervention.

Why it matters

Potential benefit: If successful, the findings could allow pregnant women to take fewer separate pills each day, improving adherence while maintaining healthy hemoglobin and iron levels for mothers and babies.

How similar studies have performed: Multiple micronutrient supplementation has shown benefits for birth outcomes, but studies examining long-term effects of taking calcium and iron together in pregnancy are limited and mostly based on short-term meal studies rather than randomized antenatal trials.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Burkina Faso: married or unmarried pregnant women aged ≥15 years
* Pakistan: married pregnant women ≥18 years
* Willing to receive all antenatal care at a study clinic
* Willing to stop iron folic acid supplementation to receive the study intervention
* Hb ≥70 g/L
* 6\<20 weeks of gestation based on fetal ultrasound

Exclusion Criteria:

* Burkina Faso: \<15 years or unmarried pregnant women \<18 years without consent from parent / guardian
* Pakistan: pregnant women \<18 years.
* Unwilling to receive antenatal care at a study clinic
* Unwilling to stop iron folic acid supplementation to receive the study intervention
* Hemoglobin \< 70 g/L
* \<6 weeks of gestation or ≥20 weeks of gestation
* Non-viable or extrauterine pregnancy
* Any contraindications to study supplements

Where this trial is running

Ouagadougou and 1 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Anemia, Iron Deficiencycalciumironsupplementationanemiapregnancy
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.