Restoring fertility by autografting frozen immature testicular tissue

Fertility Restoration With Autotransplantation of Cryopreserved Immature Testicular Tissue (ITT) Restaurer la fertilité à Partir de Tissu Testiculaire Immature (TTI) cryopréservé Par Auto-transplantation du Tissu

NA · Cliniques universitaires Saint-Luc- Université Catholique de Louvain · NCT07542626

This pilot trial will try transplanting a man's own immature testicular tissue, frozen during childhood, back into adult men with azoospermia who want to have children.

Quick facts

PhaseNA
Study typeInterventional
Enrollment20 (estimated)
Ages18 Years to 40 Years
SexMale
SponsorCliniques universitaires Saint-Luc- Université Catholique de Louvain (other)
Locations1 site (Brussels)
Trial IDNCT07542626 on ClinicalTrials.gov

What this trial studies

The procedure returns cryopreserved immature testicular tissue collected before gonadotoxic treatment in childhood into adult patients with proven azoospermia and a desire for biological children. Surgical sperm retrieval is attempted first and, if unsuccessful, autologous transplantation of the thawed tissue is performed during the same operation. Candidates are selected after completion of cancer therapy, medical clearance, and multidisciplinary review to ensure negligible risk of cancer-cell contamination. The single-center program includes post-operative follow-up to monitor safety and signs of renewed spermatogenesis.

Who should consider this trial

Good fit: Adult men who cryopreserved immature testicular tissue before gonadotoxic therapy, have completed treatment, have confirmed azoospermia, desire children, and have minimal risk of tissue contamination.

Not a fit: Patients whose stored tissue carries a significant risk of cancer cell contamination, who cannot undergo general anesthesia, or whose transplanted tissue fails to restart sperm production are unlikely to benefit.

Why it matters

Potential benefit: If successful, the procedure could restore sperm production and offer a path to biological fatherhood for men who froze testicular tissue as boys.

How similar studies have performed: While cryopreservation of immature testicular tissue has been practiced for years and animal studies show restored spermatogenesis, autologous testicular tissue transplantation in humans remains largely experimental with limited outcome data.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria: all are required

* patients who have completed their gonadotoxic treatment
* patients who are in good health after care of the primary disease based on hemato-oncologist input
* patients with proven azoospermia in adulthood and child's wish requesting transplantation
* patients who cryopreserved and stored their immature testicular tissue (in conformity with regulatory requirements)
* in case of prior hematological malignant disease or metatstatic cancer, if the multidisciplinary assesment considers the risk of cancer cell contamination is negligible

Exclusion Criteria:

* patients for whom the cryostored tissue presents a non-negligible risk of cancer cells contamination
* patients presenting with a contraindication for general anethesia

Where this trial is running

Brussels

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.

View on ClinicalTrials.gov →

Conditions: Azoospermia or Severe Oligozoospermia, Infertility, Autologous Transplantation, autotransplantation of immature testicular tissue, fertility preservation, prepubertal boys, cryopreserved testicular tissue, prepubertal testicular tissue

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.