Reduced-intensity donor stem cell transplant with treosulfan/fludarabine and post-transplant cyclophosphamide for leukemia and MDS

MT2025-35 Allogeneic Hematopoietic Stem Cell Transplantation Using Reduced Intensity Conditioning Treosulfan and Fludarabine, With Post-Transplant Cytoxan (PTCy) for the Treatment of Hematological Diseases

Phase 2 Interventional Masonic Cancer Center, University of Minnesota · NCT07493538

This trial tests a lower-intensity donor stem cell transplant using treosulfan plus fludarabine with post-transplant cyclophosphamide to try to reduce toxicity and prevent graft-versus-host disease in people aged 2–75 with acute leukemias or myelodysplastic syndromes.

Quick facts

PhasePhase 2
Study typeInterventional
Enrollment132 (estimated)
Ages2 Years to 75 Years
SexAll
SponsorMasonic Cancer Center, University of Minnesota Academic / other
Drugs / interventionscyclophosphamide
Locations1 site (Minneapolis, Minnesota)
Trial IDNCT07493538 on ClinicalTrials.gov

What this trial studies

This Phase II protocol gives a treosulfan (36 g/m2) plus fludarabine preparative regimen followed by allogeneic stem cell infusion from related, unrelated, or haploidentical family donors. Post-transplant graft-versus-host disease prophylaxis uses cyclophosphamide 40 mg/kg on specified post-transplant days along with tacrolimus and mycophenolate mofetil. The study includes patients age 2–75 with adequate organ function and no uncontrolled infections or active CNS malignancy. Outcomes will include engraftment, transplant-related toxicity, and incidence of acute and chronic GVHD.

Who should consider this trial

Good fit: Ideal candidates are patients aged 2–75 with AML, acute leukemia, or MDS who have an appropriate related, unrelated, or haploidentical donor and adequate liver, kidney, cardiac, and pulmonary function without uncontrolled infection.

Not a fit: Patients with decompensated liver failure, severe cardiac or pulmonary dysfunction, active uncontrolled infections or CNS malignancy, untreated HIV, pregnancy or breastfeeding, very recent myeloablative transplant, or CML in blast crisis are unlikely to be eligible or to benefit.

Why it matters

Potential benefit: If successful, the regimen could provide effective donor transplants with lower conditioning toxicity and less graft-versus-host disease, making transplant an option for older or frailer patients.

How similar studies have performed: Similar reduced-intensity treosulfan/fludarabine regimens have shown promising engraftment and lower toxicity in prior reports, and post-transplant cyclophosphamide is an established approach to reduce graft-versus-host disease.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Patients 2-75 years of age
* ≤7 5 years of age: Karnofsky score ≥ 70% (≥ 16 years) or Lansky play score ≥ 50 (\< 16 years) with appropriate organ criteria as below (in other inclusion criteria)
* 5/6 or 6/6 related donor, OR a 5-8/8 HLA-A, B, C, DRB1 allele match unrelated donor, OR a haplotype (at least 5/10) related donor
* adequate liver (no decompensated liver failure, Child Pugh A, AST/ALT \<5X ULN) and renal function (creatinine \<2.0)
* absence of decompensated congestive heart failure, or uncontrolled arrhythmia and left ventricular ejection fraction ≥ 40%
* DLCO FEV1, FVC ≥ 40% predicted, and absence of O2 requirement

Exclusion Criteria:

* Pregnant or breastfeeding
* Evidence of untreated/uncontrolled HIV infection
* Untreated active serious infection
* Active CNS malignancy
* CML in blast crisis not in a complete remission by abnormal blast count.
* Less than 3 months since prior myeloablative transplant

Where this trial is running

Minneapolis, Minnesota

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions AMLMDSAcute LeukemiaAcute Myeloid LeukemiaMyelodysplastic Syndromes
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.