Presence of neurotrophic growth factors in breast milk

Evaluation of Neurotrophic Growth Factors in Breast Milk at Two Years of Age and Beyond

Observational Istanbul University · NCT05943847

This study is testing whether certain brain-supporting proteins are still found in the breast milk of mothers with toddlers older than 18 months to see how they might help with brain development.

Quick facts

Study typeObservational
Enrollment25 (estimated)
Ages18 Years and up
SexFemale
SponsorIstanbul University Academic / other
Locations1 site (Istanbul)
Trial IDNCT05943847 on ClinicalTrials.gov

What this trial studies

This observational study aims to investigate the presence of neurotrophic factors, specifically Brain-Derived Neurotrophic Factor (BDNF), S100B protein, and glial cell line-derived neurotrophic factor (GDNF), in the breast milk of mothers with 18-month-old children. It will collect breast milk samples from 25 mothers at three different time points: when their child is 18 months, 24 months, and after 25 months. The study seeks to determine whether these neurotrophic factors persist in breast milk beyond the age of 2 years, contributing to our understanding of their role in infant brain development.

Who should consider this trial

Good fit: Ideal candidates for this study are mothers who have exclusively breastfed their infants for the first six months and are participating in the 18-month follow-up.

Not a fit: Mothers of infants who were not exclusively breastfed in the first six months or those with certain medical conditions may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could enhance our understanding of the nutritional and developmental benefits of breast milk for infants beyond the age of two.

How similar studies have performed: While the presence of neurotrophic factors in breast milk has been previously demonstrated in younger infants, this study's focus on older infants is relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Mothers who applied for the 18th-month follow-up of their exclusively breastfed baby for the first 6 months agreed to participate in the study.

Exclusion Criteria:

* Mothers of infants who were not exclusively breastfed in the first six months
* Mothers with infection, metabolic disease, central nervous system, breast disease or pregnancy
* Mothers of infants with infection, metabolic disease, malnutrition and central nervous system disease
* Mothers who stop breastfeeding before 25 months

Where this trial is running

Istanbul

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Breast Milk CollectionBreast milkBDNFGDNFS100B
Last reviewed 2026-06-10 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.