Noninvasive blood miRNA test to screen for multiple gastrointestinal cancers.

A Noninvasive and Screening miRNA Signature for Gastrointestinal Cancer

Observational City of Hope Medical Center · NCT07224750

This project will test whether a blood-based microRNA panel can detect several types of gastrointestinal cancer in adults using stored blood samples.

Quick facts

Study typeObservational
Enrollment1000 (estimated)
Ages18 Years and up
SexAll
SponsorCity of Hope Medical Center Academic / other
Drugs / interventionschemotherapy
Locations1 site (Duarte, California)
Trial IDNCT07224750 on ClinicalTrials.gov

What this trial studies

This retrospective international, multi-center analysis will use archived peripheral blood samples from adults with major gastrointestinal cancers (including HCC, CCA, PDAC, ESCC, GC, and CRC) and non-malignant controls. Small RNA sequencing will generate circulating miRNA expression profiles followed by preprocessing, normalization, and batch-effect correction to harmonize data across cohorts and platforms. Machine-learning feature selection and classifier development (for example LASSO, mRMR, SVM, Random Forest, XGBoost) will be used to derive a compact miRNA panel that distinguishes cancer from non-cancer and to explore tumor-origin signals. The resulting signature will undergo multi-center training and validation to test robustness across geographic regions, sequencing platforms, and clinical demographics.

Who should consider this trial

Good fit: Adults (≥18) with confirmed diagnoses of major gastrointestinal cancers (HCC, cholangiocarcinoma, PDAC, ESCC, gastric cancer, colorectal cancer) or non-cancer controls who have available, high-quality archived peripheral blood samples and de-identified clinical data are ideal candidates.

Not a fit: People without available or sufficient-quality archived blood samples, those with other active malignancies, or patients needing immediate diagnostic results are unlikely to benefit from participation in this retrospective analysis.

Why it matters

Potential benefit: If successful, this could lead to a simple blood test that detects multiple gastrointestinal cancers earlier, potentially expanding treatment options and improving outcomes.

How similar studies have performed: Prior smaller studies have shown circulating miRNAs can distinguish some cancers, but large multi-center, multi-cancer miRNA panels with rigorous external validation remain limited and unproven at scale.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

1. Adults aged 18 years or older at the time of blood sample collection.
2. Patients with a confirmed diagnosis of one of the following gastrointestinal cancers: Hepatocellular carcinoma (HCC), Cholangiocarcinoma (CCA), Pancreatic ductal adenocarcinoma (PDAC), Esophageal squamous cell carcinoma (ESCC), Gastric cancer (GC), Colorectal cancer (CRC), Non-cancer control participants, including healthy volunteers or patients with benign gastrointestinal conditions.
3. Availability of retrospective blood samples collected according to institutional protocols.
4. Willingness to allow use of de-identified clinical and demographic data for research purposes.

Exclusion Criteria:

* other active malignancies; insufficient sample quality/volume; recent chemotherapy/radiotherapy/surgery; any condition preventing reliable participation.

Where this trial is running

Duarte, California

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Hepatocellular CarcinomaCholangiocarcinomaPancreatic Ductal AdenocarcinomaEsophageal Squamous Cell CarcinomaGastric CancerColorectal Cancer ScreeningNoninvasive screeningCirculating miRNA
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.