New ALPCO blood tests to detect syphilis antibodies

ALPCO Syphilis-T and Syphilis-NT CLIA Kits: Detection of Antibodies to Treponema Pallidum and Against Lipoidal Antigens in Human Serum and Plasma

Observational American Laboratory Products Company · NCT07089836

This will test two ALPCO blood tests that look for treponemal and non-treponemal antibodies in people screened for or suspected of having syphilis, including pregnant and HIV-positive individuals.

Quick facts

Study typeObservational
Enrollment1000 (estimated)
Ages13 Years and up
SexAll
SponsorAmerican Laboratory Products Company Industry-sponsored
Locations6 sites (Lancaster, California and 5 other locations)
Trial IDNCT07089836 on ClinicalTrials.gov

What this trial studies

This multicenter, prospective specimen-collection effort will collect blood from people undergoing routine syphilis testing, those at high risk, people living with HIV, pregnant individuals, and healthy volunteers. Samples will be run on the automated KleeYa platform using the ALPCO Syphilis-T (treponemal) and Syphilis-NT (non-treponemal) CLIA kits. Results from the investigational kits will be compared to validated, FDA‑cleared treponemal and non‑treponemal reference assays to determine agreement. The primary goals are to measure positive and negative percent agreement and demonstrate the kits meet prespecified performance thresholds.

Who should consider this trial

Good fit: Ideal candidates are people aged 13 and older who are undergoing or consenting to routine syphilis testing, including pregnant individuals, people living with HIV, those with suspected infection, and healthy volunteers willing to give blood.

Not a fit: People who cannot provide blood, need immediate diagnostic results for treatment decisions, or are in the very early window period before antibodies form are unlikely to get direct clinical benefit from participation.

Why it matters

Potential benefit: If the kits meet performance targets, they could provide faster, automated, and reliable detection of syphilis antibodies to improve screening and diagnosis, especially in pregnant and HIV-positive patients.

How similar studies have performed: Comparable treponemal and non-treponemal immunoassays on automated platforms are widely used and have generally shown good performance, though these specific ALPCO kits are being prospectively validated.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Male, Female, or Other(s)
* Patients undergoing routine syphilis testing (asymptomatic at risk, suspected with symptoms, prior history of syphilis, high risk for syphilis) or previously diagnosed with syphilis through a test performed in an accredited laboratory.
* Participants must be willing to provide the required blood samples for the clinical study, in addition to any samples requested by their physician.
* Pregnant individuals and children (under 22): 1 clinical study tube of blood.
* All other participants (excluding pregnant individuals and children): 2 clinical study tubes of blood.
* Participants must be able and willing to sign the informed consent form (ICF).
* Participants aged 13 to 17 will be asked to sign an assent form. Written parental or guardian consent will also be required prior to enrollment. The assent form will use age-appropriate language and be reviewed with the participant before any procedures.

Exclusion Criteria:

* Patients currently being treated with antibiotics or completed a course of antibiotics within the past 30 days
* Patients with pre-existing conditions that would make blood collection difficult or harmful
* If the samples are not collected in strict accordance with the established sampling protocol, as determined by the study team.
* Any medical, psychological, or social condition that, in the judgment of the investigator, could interfere with the participant's ability to comply with study requirements, or pose a risk to their safety, or compromise the integrity of the study.

Where this trial is running

Lancaster, California and 5 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Treponema PallidumTreponema Pallidum InfectionSyphilis InfectionSerumPlasmaBlooddrawSexually Transmitted InfectionNon Treponema
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.