Morus nigra (black mulberry) mouthwash for gingivitis

Evaluation of the Efficacy of Morus Nigra Mouthwash as an Adjunct to Scaling and Root Planing in the Treatment of Gingivitis: A Randomized Controlled Trial

Not applicable Interventional Inonu University · NCT07499596

This trial tests whether using a Morus nigra (black mulberry) mouthwash twice daily for 14 days after scaling and root planing helps reduce gum inflammation and bacterial load in people with generalized gingivitis.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment30 (estimated)
Ages18 Years to 65 Years
SexAll
SponsorInonu University Academic / other
Locations1 site (Battalgazi, Malatya)
Trial IDNCT07499596 on ClinicalTrials.gov

What this trial studies

This randomized, controlled trial will enroll 30 patients with generalized gingivitis and compare standard non-surgical periodontal treatment (scaling and root planing, SRP) alone versus SRP plus a Morus nigra mouthwash used twice daily for 14 days. Clinical measures like Gingival Index and Plaque Index will be recorded at baseline, 1 month, and 3 months. Biochemical analysis will measure cytokine levels (TNF-alpha, IL-1beta, IL-10) in gingival crevicular fluid by ELISA, and microbiological analysis of subgingival plaque will be done by real-time PCR. The trial is single-center at Inonu University Faculty of Dentistry and focuses on short-term adjunctive effects of the mouthwash following SRP.

Who should consider this trial

Good fit: Ideal candidates are systemically healthy adults with generalized gingivitis, at least 20 natural teeth, who are non-smokers and can attend visits at the dental center and provide informed consent.

Not a fit: Patients with periodontitis, systemic diseases (like diabetes or cardiovascular disease), recent antibiotic or anti-inflammatory use, smokers, pregnant or lactating individuals, or those allergic to Morus nigra are unlikely to benefit or be eligible.

Why it matters

Potential benefit: If successful, the mouthwash could provide an easy adjunct to SRP that reduces gum inflammation and lowers harmful subgingival bacteria.

How similar studies have performed: Preclinical and small clinical reports suggest Morus extracts have anti-inflammatory and antimicrobial properties, but clinical evidence specifically for Morus nigra mouthwash as an adjunct in gingivitis is limited.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Diagnosis of generalized gingivitis (Gingival Index \> 1).
* Presence of at least 20 natural teeth.
* Systemically healthy individuals.
* Ability to provide informed consent.

Exclusion Criteria:

* Smoking or use of tobacco products.
* Presence of periodontitis (no clinical attachment loss).
* Use of antibiotics or anti-inflammatory drugs in the last 6 months.
* Pregnancy or lactation.
* History of allergy to Morus nigra or mouthwash components.
* Systemic diseases (Diabetes, cardiovascular diseases, etc.).

Where this trial is running

Battalgazi, Malatya

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions GingivitisMorus nigraMouthwashCytokines
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.