Injection of targeted T cells for patients with refractory central nervous system lymphoma

A Early Phase 1 Clinical Trial to Evaluate the Safety and Efficacy of Human CD19-CD22 Targeted T Cells Injection for Subjects with Central Nervous System Involvement of Refractory/Relapsed B Cell Malignancies

This study is testing a new treatment using specially targeted T cells for patients with tough-to-treat central nervous system lymphoma to see if it helps them feel better.

Quick facts

• Phase: Early Phase 1
• Study type: Interventional
• Enrollment: 10 (estimated)
• Ages: 18 Years to 70 Years
• Sex: All
• Sponsor: Hrain Biotechnology Co., Ltd. (Industry-sponsored)
• Drugs / interventions: CAR-T, chemotherapy
• Locations: 1 site (Nanchang, Jiangxi)
• Trial ID: NCT05651178 on ClinicalTrials.gov

What this trial studies

This open-label, dose-escalation trial investigates the safety, tolerability, and pharmacokinetic/pharmacodynamics of Human CD19-CD22 Targeted T Cells administered both intravenously and intrathecally. Eligible participants are patients with refractory or relapsed central nervous system involvement of B cell malignancies, who will undergo preconditioning chemotherapy before receiving the T cell infusion. Following the infusion, participants will be monitored for adverse events and the efficacy of the treatment. The study aims to provide preliminary efficacy data while ensuring patient safety throughout the process.

Who should consider this trial

Good fit: Ideal candidates are adults aged 18 to 70 with CD19 or CD22 positive B cell malignancies and central nervous system involvement.

Not a fit: Patients with non-B cell malignancies or those with severe comorbidities affecting treatment eligibility may not benefit from this study.

Why it matters

Potential benefit: If successful, this treatment could offer a new therapeutic option for patients with difficult-to-treat central nervous system lymphomas.

How similar studies have performed: While this approach is innovative, similar CAR-T cell therapies have shown promise in other settings, indicating potential for success.

Eligibility criteria

Inclusion Criteria:Subjects must meet all of the following criteria to be enrolled:

* B cell malignancies patients with CD19 or CD22 positive, or CD19 and CD22 positive，include B cell acute lymphoblastic leukemia relapsed with central nervous system invasion and refractory/relapsed B cell lymphoma of central nervous system;
* 18 to 70 years old (including cut-off value), Male and female;
* Expected survival \> 12 weeks;
* ECOG score 0-2;
* Definitive diagnosed as central nervous system of B cell malignancies by Cerebrospinal fluid and/or MRI, PET/CT or other imaging examinations;
* The venous access required for collection can be established and leukapheresis can be carried according to the judgement of investigators;
* Liver, kidney and cardiopulmonary functions meet the following requirements:

  1. The detection value of Creatinine within the normal range;
  2. Left ventricular ejection fraction \> 50%;
  3. Baseline oxygen saturation \> 92%;
  4. Total bilirubin ≤ 2×ULN;
  5. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ≤ 2.5×ULN;
* Able to understand and sign the Informed Consent Document.

Exclusion Criteria:Any one of the following conditions cannot be selected as a subject:

* Malignant tumors other than B cell malignancies within 5 years prior to screening, in addition to adequately treated cervical carcinoma in situ, basal cell or squamous cell skin cancer, localized prostate cancer after radical resection, ductal carcinoma in situ after radical resection and thyroid cancer after radical resection;
* Subjects with positive Hepatitis B surface antigen (HBsAg) or Hepatitis B core antibody (HBcAb) and peripheral blood hepatitis B virus (HBV) DNA titer detection higher than or equal to the lower limit of detection; hepatitis C virus (HCV) antibody positive and peripheral blood HCV RNA positive; human immunodeficiency virus (HIV) antibody positive; syphilis detection positive;
* Any instability of systemic disease, including but not limited to unstable angina, cerebrovascular accident, or transient cerebral ischemic (within 6 months prior to screening), myocardial infarction (within 6 months prior to screening), congestive heart failure (New York heart association (NYHA) classification ≥ III), need drug therapy of severe arrhythmia, liver, kidney, or metabolic disease;
* Active or uncontrollable infection requiring systemic therapy within 14 days prior to enrollment;
* Pregnant or lactating woman, and female subject who plans to have a pregnancy within 1 year after cell transfusion, or male subject whose partner plans to have a pregnancy within 1 year after cell transfusion;
* Received CAR-T treatment or other gene therapies before enrollment;
* Subjects who are receiving systemic steroid treatment and requiring long-term systemic steroid treatment during the treatment as determined by the investigator before screening (except inhalation or topical use); And subjects treated with systemic steroids (except inhalation or topical use) within 72h prior to cell infusion;
* The investigators consider other conditions unsuitable for enrollment.

Where this trial is running

Nanchang, Jiangxi

• The Second Affiliated Hospital of Nanchang University — Nanchang, Jiangxi, China (Recruiting)

Study contacts

• Principal investigator: Qingming Wang, M.D. — Second Affiliated Hospital of Nanchang University
• Study coordinator: Xuedong Sun, M.D.

Conditions Central Nervous System Lymphoma; CD19-CD22; CAR-T; Central nervous system leukemia/lymphoma; Refractory/Relapsed