Impact of Centrifuge Speed on Saliva Protein Levels in Smokers

Evaluating the Effect of Smoking Amount on Determining Salivary High Sensitive C-Reactive Protein(Hs-CRP) Values by Centrifuging the Saliva of the Same Individuals at Different Rpm and Time.

Not applicable Interventional Izmir Katip Celebi University · NCT06561971

This study tests how different speeds of a machine that spins saliva samples can change the levels of a specific protein in the saliva of smokers and non-smokers to better understand the effects of smoking on oral health.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment90 (estimated)
Ages18 Years to 30 Years
SexAll
SponsorIzmir Katip Celebi University Academic / other
Locations1 site (Izmir, Çiğli)
Trial IDNCT06561971 on ClinicalTrials.gov

What this trial studies

This study investigates how different centrifuge speeds affect the levels of high sensitive C-reactive protein (hs-CRP) in saliva among individuals with varying smoking habits. It involves collecting saliva samples from 90 healthy participants categorized as non-smokers, light smokers, and heavy smokers. The samples will be centrifuged at different speeds to determine the optimal conditions for measuring hs-CRP levels and to explore any correlations with clinical parameters related to smoking. The study aims to enhance understanding of the biochemical effects of smoking on oral health.

Who should consider this trial

Good fit: Ideal candidates include systemically healthy individuals who are either non-smokers, light smokers (less than 10 cigarettes per day), or heavy smokers (more than 10 cigarettes per day) with no recent periodontal treatment.

Not a fit: Patients with oral or systemic diseases, those on regular medication, or individuals who have received recent periodontal treatment will not benefit from this study.

Why it matters

Potential benefit: If successful, this study could provide insights into the relationship between smoking and inflammation, potentially guiding better oral health management for smokers.

How similar studies have performed: While there may be studies examining hs-CRP levels in relation to smoking, the specific focus on centrifuge speeds and saliva analysis in this context appears to be a novel approach.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Systemically healthy
* Periodontally clinical healthy
* At least 20 permanent teeth in the mouth
* No medication for continuous use
* Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
* Not in pregnancy or lactation period.
* Not received periodontal treatment in the last 6 months

  * For the non-smoker group; person who does not smoke tobacco
  * For the light-smoker group; person who smokes less than 10 cigarettes a day
  * For heavy-smoker group; person who smokes more than 10 cigarettes a day

Exclusion Criteria:

* Any oral or systemic disease
* Regularly using a systemic medication
* During pregnancy or lactation
* Received periodontal treatment within the last 6 months.
* Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
* Individual with periodontal disease

Where this trial is running

Izmir, Çiğli

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Smoking
Last reviewed 2026-06-10 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.