How blood flow restriction leg training affects muscles and blood markers
Official title: The Effects of Training History on Physiological and Biochemical Responses in Blood Flow Restriction Strength Training
Run by Afyonkarahisar Health Sciences University · NCT07340385 · Data updated 2026-11-12
This project tests if six weeks of blood flow restriction leg training changes muscle size and blood biomarkers in healthy adults aged 18–40, including both people new to strength training and experienced athletes.
Quick facts
| Study type | Observational |
|---|---|
| Ages | 18 Years to 40 Years |
| Sex | Men only |
| Enrollment | 30 (estimated) |
| Sponsor | Afyonkarahisar Health Sciences University Academic / other |
| Locations | 1 site (Afyonkarahisar, Merkez) |
| Status | Recruiting |
| Started | January 20, 2026 |
| Main results expected | January 20, 2027 |
| Study ends | March 20, 2027 |
| Registry record updated | February 13, 2026 |
| Trial ID | NCT07340385 on ClinicalTrials.gov |
Is this trial a fit for you?
✓Likely a good fit
Ideal candidates are healthy men and women aged 18–40 with no lower-extremity orthopedic issues, who are either new to strength training or have been doing strength work at least two days per week for the past year.
✕Probably not a fit
People with hypertension, cardiovascular or peripheral vascular disease, deep vein thrombosis, neurological disorders, obesity, diabetes, current cancer, or those outside the 18–40 age range are unlikely to be eligible or benefit from participation.
A plain-English reading of the study's rules. Only the study team can confirm whether you qualify.
What this trial studies
Thirty healthy adults aged 18 to 40 will complete a twice-weekly, six-week exercise program in which an adjustable inflatable cuff is applied to the dominant thigh while all exercises are performed with both legs. Cuff pressure will be individualized for safety and comfort, and participants will include both individuals new to strength training and athletes who train regularly. Researchers will collect short-term and longer-term physiological and biochemical measurements such as oxidative stress markers, growth factors, other blood biomarkers, and muscle thickness before and after the program. The protocol is designed to compare responses across different training backgrounds and load levels.
Why it matters
Potential benefit: If successful, this work could show a way to boost muscle adaptation with lower loads or provide safer, targeted training protocols for healthy adults and athletes.
How similar studies have gone: Previous research on blood flow restriction training has often shown increased muscle size and growth-factor responses at low loads, though findings on oxidative stress and some biochemical markers have been mixed.
Who can join: the full rules
Show full inclusion / exclusion criteria
Inclusion Criteria: * Voluntary participation of healthy adults * Age between 18 and 40 years * No history of orthopedic pathology or current symptoms affecting the lower extremities * For individuals with prior training experience: engagement in strength training at least two days per week for the past year across different sports disciplines * For individuals new to strength training: no regular participation in strength training within the past six months Exclusion Criteria: * History of hypertension, cardiovascular diseases, peripheral vascular diseases, deep vein thrombosis, neurological disorders, obesity, or a diagnosis of diabetes * Current or previous diagnosis of cancer * Individuals who do not consent to participate in the study
Where it's running
-
Afyonkarahisar Health Sciences University
Afyonkarahisar, Merkez, Turkey (Türkiye) Recruiting
Study contacts
- Study coordinator: Dilara KARA
- Email: dilaradonder@gmail.com
- Phone: +90 530 237 1991
Similar studies
Other studies for the same condition or treatment. Recruiting studies are listed first.
-
Trained vs Untrained: How Endurance Status Affects Lung Blood Flow During Blood Volume Changes
This study tests whether highly trained athletes show different lung blood flow responses when blood return to the heart changes, compared with untrained individuals.
-
How people use beans, peas, and lentils in meals
This project will test how Americans who regularly cook choose beans, peas, and lentils when building mixed dishes and how they feel about eating them.
-
BLa80 and LRa05 probiotics and blood metabolite changes in healthy adults
This test will see if taking the probiotics BLa80 or LRa05 changes how healthy adults break down and absorb food, as shown by metabolites in the blood.
-
Heel-lift insole effects on lower limb and lower back motion
This study will test whether wearing a heel lift insole changes muscle activity and movement angles of the lower limbs and lower back in healthy young adults.
-
Trans-spinal magnetic stimulation to modulate cortical excitability in healthy adults
We test whether different frequencies and intensities of trans‑spinal magnetic stimulation change motor cortex excitability in healthy adults aged 18–40.
-
Peripheral magnetic stimulation effects on motor‑cortex excitability in healthy adults
This project will try different peripheral magnetic stimulation settings to see if they change motor‑cortex excitability in healthy adults aged 18–40.
How to take part
- Review the eligibility criteria above with your treating physician.
- Check the official listing on ClinicalTrials.gov for the latest contact details and recruitment status.
- Contact the study coordinator or principal investigator to ask for pre-screening. Pre-screening is free and never obligates you to enroll.