How 24-hour sleep loss affects people with different APOE genes

Is this trial a fit for you?

A plain-English reading of the study's rules. Only the study team can confirm whether you qualify.

What this trial studies

This randomized controlled trial will enroll 60 healthy adults aged 18–40 and stratify them by APOE genotype (ε3/3, ε4 carriers, and ε2 carriers). Within each genotype stratum participants will be randomly assigned to either 24 hours of acute sleep deprivation or to normal sleep. Blood samples will be collected before and after the intervention to measure plasma Aβ42 and a range of multi-omics biomarkers, with the primary outcome being the change in plasma Aβ42 the day after randomization. The design tests whether a single night of sleep loss produces different molecular responses depending on APOE genotype.

Why it matters

Who can join: the full rules

Inclusion Criteria:

* Age 18-40 years, gender not limited
* Healthy (with no clinically significant abnormal findings in the physical examination report or self-reporting as healthy) and not on medications
* Cognitively normal (Mini-Mental State Examination (MMSE) score \> 28)
* Sleep duration of 7-9 hours per night, good sleep quality (Pittsburgh Sleep Quality Index (PSQI) ≤ 5 points)
* Written informed consent, voluntarily participate in this study, and be able to cooperate with the physician to complete the clinical study

Exclusion Criteria:

* Presence of day-night sleep reversal
* Shift work within the past 6 months
* Travel across time zones or experience of jet lag within the past three weeks
* Current smoking or nicotine use; alcohol consumption exceeding five standard units per week (one standard alcohol unit is defined as 10 mL \[or 8 g\] of pure alcohol)
* Consumption of strong tea, coffee, or caffeine-containing foods and beverages within one week before study participation
* Family history of early-onset dementia
* Self-Rating Depression Scale (SDS) score ≥ 53, Self-Rating Anxiety Scale (SAS) score ≥ 50
* Female participants who are currently pregnant or breastfeeding
* Individuals who need to drive or operate vehicles or machinery during the study period

Where it's running

• Guangdong Provincial Hospital of Chinese Medicine
  Guangzhou, Guangdong, China Recruiting

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How to take part

1. Review the eligibility criteria above with your treating physician.
2. Check the official listing on ClinicalTrials.gov for the latest contact details and recruitment status.
3. Contact the study coordinator or principal investigator to ask for pre-screening. Pre-screening is free and never obligates you to enroll.