Giving protective particles from donor breast milk to very premature babies

Administration of Extracellular Vesicles Isolated From Donor Human Milk as a Dietary Supplement for the Prevention of Necrotizing Enterocolitis in Preterm Infants

Observational Instituto de Investigacion Sanitaria La Fe · NCT07402083

This pilot will try giving extracellular vesicles from donor human milk to babies born before 32 weeks to see if it helps feeding and protects their intestines.

Quick facts

Study typeObservational
Enrollment20 (estimated)
Ages0 Days to 14 Days
SexAll
SponsorInstituto de Investigacion Sanitaria La Fe Academic / other
Locations1 site (Valencia, Valencia)
Trial IDNCT07402083 on ClinicalTrials.gov

What this trial studies

AdVEMPrem is a prospective, single-arm pilot enrolling 20 infants born before 32 weeks' gestation who are less than 14 days old and hospitalized in the NICU. All enrolled infants will receive oral supplementation with extracellular vesicles (EVs) isolated and quality-controlled from pasteurized donor human milk during their hospital stay. Researchers will monitor feeding tolerance, safety, and clinical signs of necrotizing enterocolitis, and will perform biochemical and lipid analyses of milk and EVs for exploratory correlations. The protocol focuses on feasibility and safety and includes standardized methods for EV isolation and product characterization.

Who should consider this trial

Good fit: Ideal candidates are neonates born before 32 weeks' gestation, enrolled within 0–14 days of life, admitted to the NICU, and whose parents provide informed consent.

Not a fit: Infants with major congenital or chromosomal anomalies, severe gastrointestinal malformations, or conditions incompatible with enteral feeding are unlikely to receive benefit from this intervention.

Why it matters

Potential benefit: If successful, EV supplementation could improve feeding tolerance and reduce the risk or severity of necrotizing enterocolitis in very preterm infants.

How similar studies have performed: Preclinical laboratory and animal studies suggest milk-derived EVs reduce inflammation and support gut development, but clinical evidence in very preterm infants is currently lacking, making this among the first human pilot applications.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Preterm infants born at \<32 weeks gestational age
* Age between 0 and 14 days of life at enrollment
* At risk of developing necrotizing enterocolitis (NEC)
* Written informed consent obtained from parent(s) or legal guardian(s)

Exclusion Criteria:

* Major congenital anomalies or chromosomal abnormalities
* Severe gastrointestinal malformations (e.g., gastroschisis, intestinal atresia)
* Conditions incompatible with enteral feeding or EV supplementation
* Participation in another interventional clinical trial

Where this trial is running

Valencia, Valencia

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Very Preterm InfantsPreterm birthDonor human milkExtracellular vesiclesFeeding toleranceNecrotizing enterocolitisNeurodevelopmentDonor Human Milk Extracellular Vesicle
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.