Evaluating the effectiveness of Tenecteplase for treating certain ischemic strokes

A Phase III, Randomized, Multi-center Clinical Trial That Will Examine Whether Treatment With Intravenous TNK is Superior to Placebo in Patients Who Suffer a Non-large Vessel Occlusion Ischemic Stroke Within 4.5-12 Hours From Time Last Seen Well

This study is testing if a new stroke treatment called Tenecteplase can help people who had certain types of strokes feel better compared to a placebo.

Quick facts

• Phase: Phase 3
• Study type: Interventional
• Enrollment: 466 (estimated)
• Ages: 18 Years and up
• Sex: All
• Sponsor: Hospital Moinhos de Vento (Academic / other)
• Locations: 15 sites (Porto Alegre, Rio Grande do Sul and 14 other locations)
• Trial ID: NCT05199662 on ClinicalTrials.gov

What this trial studies

This phase III clinical trial investigates whether intravenous Tenecteplase (TNK) is more effective than a placebo in treating patients with non-large vessel occlusion ischemic strokes occurring 4.5 to 12 hours after the last time the patient was seen well. The study employs a randomized, double-blind design with a 1:1 ratio of TNK to placebo, focusing on patients who show evidence of salvageable brain tissue on imaging. A total of 466 participants will be enrolled, and they will be monitored for 90 days post-randomization to assess outcomes. The trial includes an adaptive design allowing for interim analysis to evaluate efficacy or futility based on the data collected.

Who should consider this trial

Good fit: Ideal candidates are adults aged 18 and older who have experienced an acute ischemic stroke and are ineligible for standard thrombolytic treatments due to timing and occlusion criteria.

Not a fit: Patients with significant pre-stroke functional disabilities or those who do not meet the specific imaging criteria for salvageable brain tissue may not benefit from this study.

Why it matters

Potential benefit: If successful, this treatment could significantly improve outcomes for patients suffering from specific types of ischemic strokes that currently have limited treatment options.

How similar studies have performed: Previous studies have shown promise in using Tenecteplase for ischemic stroke treatment, but this specific approach is novel and has not been extensively tested.

Eligibility criteria

Inclusion Criteria:

1. Acute ischemic stroke where patient is ineligible for IV thrombolytic treatment with Alteplase due to onset \>4.5 hours and is ineligible for endovascular treatment under standard of care due to absence of proximal arterial occlusion (e.g. intracranial ICA, MCA-M1 and dominant M2 segments, and vertebrobasilar arteries).

   \* Dominant M2 segment is defined is a division supplying \>50% of the MCA territory vs co-dominant supplying 50% of the MCA territory vs non-dominant supplying \<50% of the MCA territory.
2. No significant pre-stroke functional disability (mRS ≤2).
3. Age ≥18 years (no upper age limit).
4. Clinical or imaging mismatch evidence in distal artery territories, defined as one of the following scenarios (A, B or C):

   * Scenario A - all of the following:

     * Significant cortical neurological deficit (moderate to severe afasia, moderate to severe heminegligence, severe hemianopsia) with the addition or not of motor symptoms OR any motor deficit accompanied of cortical symptoms of any severity;
     * Contrast-enhanced CT of the head or head MRI with \<50% involvement of the vascular territory corresponding to the clinical manifestation;
     * Arterial head angiotomography or arterial head angioMRI WITHOUT proximal intracranial artery occlusion that would require endovascular therapy (for example, ICA intracranial, MCA-M1 and M2 dominant segments and vertebral and basilar arteries)
   * Scenario B - all of the following:

     * NIHSS score ≥ 4 due to any neurologic deficits;
     * Non-contrast CT of the head or head MRI com \<50% involvement of the vascular territory corresponding to the clinical manifestation;
     * Arterial head angioCT or arterial head MRI WITHOUT proximal intracranial artery occlusion that would require endovascular therapy (for example, ICA intracranial, MCA-M1 and M2 dominant segments and vertebral and basilar arteries)
     * Arterial head angioCT with distal occlusion on MIP or wedge-shaped lesion on parenchymography on the source-image of angiotomography OR CT perfusion with wedge-shaped cortical lesion.
   * Scenario C - all of the following:

     * NIHSS score ≥ 4 due to any neurologic deficits;
     * The presence of a Target Mismatch defined as:

       * Ischemic Core \<50cc (defined on NCCT/CTP\* or DWI MRI) \*Volume NCCT can be used to exclude patients if the investigator believes that its volume assessment is more reliable that the CTP in any particular case.
       * Mismatch Volume (Tmax \>6sec lesion - Core volume lesion) \>10cc
       * Mismatch Ratio \>1.4
5. Patient treatable within 4.5-12 hours of symptom onset. Symptoms onset is defined as point in time the patient was last seen well (at baseline). Treatment start is defined as initiation of IV TNK or placebo infusion.

   * Patients who have woken up with the symptoms and don't have a mismatch FLAIR-DWI according to the WAKE-UP Trial image criteria will have their window considered to be \>4.5 hours. In this case, the time last seen well must have been 12 hours at most.
6. Informed consent obtained from patient or acceptable patient surrogate.

Exclusion Criteria:

1. Intracranial hemorrhage (ICH) identified by CT or MRI.
2. Rapidly improving symptoms, particularly if in the judgment of the managing clinician that the improvement is likely to result in the patient without eligibility criteria.
3. Pre-stroke mRS score of ≥ 2 (indicating previous disability)
4. Contra indication to imaging with MR or CT with contrast agents.
5. Infarct core \>1/3 MCA territory qualitatively or \>50 mL quantitatively (determined by DWI lesion on MR).
6. Participation in any investigational study in the previous 30 days
7. Any terminal illness such that patient would not be expected to survive more than 1 year).
8. Baseline platelet count \< 100.000/µL
9. Woman of childbearing potential who is known to be pregnant or who has a positive pregnancy test on admission.
10. Previous stroke within last three months.
11. Recent past history or clinical presentation of ICH, subarachnoid hemorrhage (SAH), arterio-venous (AV) malformation, aneurysm, or cerebral neoplasm other than meningioma.
12. Current use of oral anticoagulants and a prolonged prothrombin time (INR \> 1.6).
13. Use of heparin, except for low dose subcutaneous heparin, in the previous 48 hours and a prolonged partial thromboplastin time exceeding the upper limit of the local laboratory normal range
14. Use of glycoprotein IIb - IIIa inhibitors within the past 72 hours. Use of single agent oral platelet inhibitors (clopidogrel or low-dose aspirin) prior to study entry is permitted.
15. Clinically significant hypoglycemia.
16. Uncontrolled hypertension defined by a blood pressure \> 185 mmHg systolic or \>110 mmHg diastolic on at least 2 separate occasions at least 10 minutes apart, or requiring aggressive treatment to reduce the blood pressure to within these limits. The definition of "aggressive treatment" is left to the discretion of the responsible Investigator.
17. Hereditary or acquired hemorrhagic diathesis.
18. Gastrointestinal or urinary bleeding within the preceding 21 days.
19. Major surgery within the preceding 14 days which poses risk in the opinion of the Investigator.
20. Exposure to a thrombolytic agent within the previous 72 hours.
21. Subject participating in a study involving an investigational drug or device that would impact this study.

Where this trial is running

Porto Alegre, Rio Grande do Sul and 14 other locations

• Hospital Moinhos de Vento — Porto Alegre, Rio Grande do Sul, Brazil (Recruiting)
• Hospital das Clínicas Botucatu — Botucatu, Brazil (Recruiting)
• Hospital das Clínicas - UNICAMP — Campinas, Brazil (Recruiting)
• Hospital Universitário Maria Aparecida Pedrossian — Campo Grande, Brazil (Recruiting)
• Hospital das Clínicas UFPR — Curitiba, Brazil (Recruiting)
• Hospital Geral de Fortaleza — Fortaleza, Brazil (Recruiting)
• Clinica Neurologica e Neurocirurgica de Joinville — Joinville, Brazil (Recruiting)
• Hospital Metropolitano de Maceió — Maceió, Brazil (Recruiting)
• Hospital de Clínicas de Porto Alegre — Porto Alegre, Brazil (Recruiting)
• Hospital de Clínicas de Porto Alegre — Porto Alegre, Brazil (Recruiting)
• Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto - Universidade de São Paulo — Ribeirão Preto, Brazil (Recruiting)
• Hospital de Base São José do Rio Preto — São José do Rio Preto, Brazil (Recruiting)
• Hospital São Paulo — São Paulo, Brazil (Recruiting)
• Santa Casa de Misericordia de Sao Paulo — São Paulo, Brazil (Recruiting)
• Hospital Estadual Central — Vitória, Brazil (Recruiting)

Study contacts

• Principal investigator: Gisele Sampaio Silva, MD, MPH, PhD — Universidade Federal de São Paulo
• Study coordinator: Gisele Sampaio Silva, MD, MPH, PhD

Conditions Ischemic Stroke, Acute; Ischemic Stroke; Thrombolysis; Tenecteplase