Evaluating changes in gut bacteria after enema treatment for constipation in children

Evaluation of Fecal Microbiome Changes After Antegrade Continence Enema Placement and Initiation of Bowel Flush Regimen

Phase 4 Interventional Indiana University · NCT05821309

This study is testing how a special enema treatment for constipation affects the gut bacteria in children aged 2 to 18 over several months.

Quick facts

PhasePhase 4
Study typeInterventional
Enrollment65 (estimated)
Ages2 Years to 18 Years
SexAll
SponsorIndiana University Academic / other
Locations2 sites (Carmel, Indiana and 1 other locations)
Trial IDNCT05821309 on ClinicalTrials.gov

What this trial studies

This study evaluates the changes in the fecal microbiome of pediatric patients suffering from chronic functional constipation before and after the placement of a Malone Antegrade Continence Enema (MACE) and the initiation of a bowel flush regimen. Children aged 2 to 18 years will have their microbiome sequenced through stool samples collected at various intervals: before the procedure and at 1, 4, and 8 months post-treatment. The study will also randomize the bowel flush regimen between two types of PEG solutions to assess their impact on microbiome diversity. The research aims to provide insights into how these interventions affect gut health in constipated children.

Who should consider this trial

Good fit: Ideal candidates are children aged 2 to 18 years with chronic functional constipation requiring a MACE procedure and intact colonic motility.

Not a fit: Patients with underlying anatomical or pathological causes of constipation or those who have had prior gastrointestinal surgeries may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to improved management strategies for chronic constipation in pediatric patients by understanding the role of the microbiome.

How similar studies have performed: While studies on the microbiome and constipation exist, this specific approach combining MACE and microbiome analysis is relatively novel and has not been extensively tested.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Recalcitrant chronic functional constipation necessitating a MACE appendicostomy or cecostomy for treatment at Riley Hospital for Children
* Intact colonic motility as evidenced by CMS studies

Exclusion Criteria

* Underlying anatomic or pathologic etiology for constipation
* History of prior gastrointestinal surgery (excluding placement of G or GJ tubes)
* Underlying severe GI disease unrelated to the patient's chronic constipation
* Use within the past month of consent of probiotic supplements, prebiotic supplements or antibiotics

Where this trial is running

Carmel, Indiana and 1 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions ConstipationFecal microbiomeMalone Antegrade Continence EnemaMiralaxPEG 3350Antegrade Continence EnemaChronic Constipation
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.