Effects of fasting and overfeeding on metabolism in women

Thermogenic Responses to Fasting and Overfeeding in Women: a Potential Mechanism Contributing to Postmenopausal Weight Gain

Not applicable Interventional University of Colorado, Denver · NCT06610162

This study tests how fasting and overeating affect metabolism in premenopausal and postmenopausal women to see how menopause influences weight gain.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment56 (estimated)
Ages18 Years to 65 Years
SexFemale
SponsorUniversity of Colorado, Denver Academic / other
Drugs / interventionscart
Locations1 site (Aurora, Colorado)
Trial IDNCT06610162 on ClinicalTrials.gov

What this trial studies

This study aims to investigate how menopause affects weight gain by comparing the metabolic responses of premenopausal and postmenopausal women to fasting and overfeeding. Participants will undergo three conditions: eucaloric feeding, acute fasting for 24 hours, and acute overfeeding with a low-protein diet. The study will measure energy expenditure and its components using a calorimeter to understand the metabolic changes associated with menopause. The research seeks to clarify the relationship between hormonal changes and energy metabolism during this life stage.

Who should consider this trial

Good fit: Ideal candidates include premenopausal women aged 18-45 with regular menstrual cycles and postmenopausal women who have not menstruated for at least 12 months.

Not a fit: Patients with a body mass index over 30 kg/m2, diabetes, or those on estrogen-based hormonal therapy may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could provide insights into managing weight gain and metabolic health in menopausal women.

How similar studies have performed: Other studies have explored metabolic responses to dietary changes, but this specific comparison of fasting and overfeeding in relation to menopause is relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion criteria for premenopausal women

* Age 18-45 years.
* Regular menses (no missed cycles in the previous year; cycle length 25-35 d).

Inclusion criteria for postmenopausal women

* No menses for at least 12 months
* Follicular stimulating hormone (FSH) ≥50 mIU/ml.
* Women who are within 2 years of the final menstrual period are preferred

The primary exclusion criteria are:

* Body mass index \> 30 kg/m2
* Diabetes (fasting glucose \>126 mg/dL).
* Thyroid dysfunction, defined as an ultrasensitive thyroid stimulating hormone (TSH) \<0.5 or \>5.0 mU/L
* Uncontrolled hypertension defined as resting systolic BP \>150 mmHg or diastolic BP \>90 mmHg.

Additional exclusion criteria for postmenopausal women

* History or current use of estrogen-based hormonal therapy
* Women who have undergone surgical menopause.

Additional exclusion criteria for premenopausal women

* Current hormonal contraceptive use (past 6 mo.).
* Pregnant, lactating, or intention to become pregnant during the study period.

Participants who fail the thyroid and BP criteria will be considered for enrollment if they receive treatment for these conditions and are considered stable by their treating physician.

Where this trial is running

Aurora, Colorado

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Obesityenergy expenditureadaptive thermogenesismenopause
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.