Effects of Estradiol on Stress Reactivity in Women

Associations of Estradiol and Stress Reactivity in Pre- and Postmenopausal Women

Observational International Research Training Group 2804 · NCT06204016

This study is testing if taking estradiol can change how premenopausal and postmenopausal women react to stress compared to those who take a placebo.

Quick facts

Study typeObservational
Enrollment74 (estimated)
Ages18 Years to 60 Years
SexFemale
SponsorInternational Research Training Group 2804 Academic / other
Locations1 site (Tuebingen, BW)
Trial IDNCT06204016 on ClinicalTrials.gov

What this trial studies

This observational study investigates how estradiol influences stress reactivity in premenopausal and postmenopausal women. Participants will receive either estradiol valerate or a placebo and undergo a psychosocial stress task while being monitored with functional magnetic resonance imaging (fMRI). The study aims to assess changes in stress responses from subjective, physiological, and neural perspectives, focusing on the role of estradiol in modulating stress-related brain activity. By comparing the two groups, the researchers hope to clarify the hormonal effects on stress reactivity across different life stages.

Who should consider this trial

Good fit: Ideal candidates include biologically female individuals aged 18-60, either premenopausal with regular menstrual cycles or postmenopausal, who meet specific health criteria.

Not a fit: Patients with neurological or mental diseases, chronic medical conditions, or those currently pregnant or lactating may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to improved understanding and management of stress-related conditions in women, particularly around menopause.

How similar studies have performed: While the effects of estradiol on stress reactivity have been explored, this specific approach using neuroimaging and a controlled design is relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Women, biologically female (assigned sex at birth)
* normal body mass index (18-28 kg/m2)
* Caucasian
* non-smoking
* German language fluency: at least advanced technical college entrance qualification
* Naturally cycling women, older than 18 years with a regular menstrual cycle (25-35 days) and postmenopausal women up to the age of 60 will be included.

Exclusion Criteria:

* Neurological or mental disease
* Medical problems such as hormonal, metabolic, or chronic diseases (e.g., severe hypertension, diabetes, dysfunctions of the thyroid, or congestive heart failure) as well as venous thromboembolism
* Pregnancy, delivery, and lactation (current and within the last year)
* Any kind of steroid hormonal, pharmacological treatment, or psychotropic treatment in the last three months
* Shift work
* Participants engaging in competitive/extreme sports
* Contraindication for MRI

  * People with non-removable metal objects on or in the body
  * Tattoos (if not MRI-incompatible according to expert guidelines)
  * Pathological hearing or increased sensitivity to loud noises
  * Claustrophobia
  * Surgery less than three months ago
  * Neurological disease or injury
  * Moderate or severe head injury
  * Intake of antidepressants or neuroleptics
  * Restricted vision

Where this trial is running

Tuebingen, BW

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Stress vs. ControlEstradiol vs. Placebostress reactivitymood homeostasisneuroimagingmenopausemenstrual cyclesex hormones
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.