Characterizing extracellular vesicles and blood–brain barrier changes during CAR‑T therapy to understand ICANS

Temporal Characterization of Extracellular Vesicles During Cellular Therapy Using CAR-T Cells and During the Occurrence of Immune Effector Cell-Associated Neurotoxicity Syndrome

Phase 4 Interventional Centre Hospitalier Universitaire de Saint Etienne · NCT06706102

This research will try to see if tracking extracellular vesicles in blood and MRI markers of blood–brain barrier change can help detect or explain ICANS in adults receiving CAR‑T cell therapy.

Quick facts

PhasePhase 4
Study typeInterventional
Enrollment60 (estimated)
Ages18 Years and up
SexAll
SponsorCentre Hospitalier Universitaire de Saint Etienne Academic / other
Drugs / interventionsCAR-T
Locations1 site (Saint-Etienne)
Trial IDNCT06706102 on ClinicalTrials.gov

What this trial studies

VESICANS will enroll adults receiving clinically indicated CAR‑T therapy at CHU de Saint‑Etienne and collect serial blood samples before treatment, during therapy, and at the onset of any ICANS. Extracellular vesicles will be profiled using flow cytometry, electron microscopy, and nanoparticle tracking analysis, while MRI will assess blood–brain barrier integrity and neuropsychological tests will monitor cognitive and neurological status. The study will correlate temporal changes in EV populations and imaging markers with the timing and severity of ICANS. Findings aim to identify biomarkers or mechanisms that could inform earlier detection or prevention of this neurotoxicity.

Who should consider this trial

Good fit: Adults (age ≥18) who are scheduled to receive CAR‑T cell therapy at CHU de Saint‑Etienne, can give informed consent, and are affiliated with a social security system are ideal candidates.

Not a fit: Patients who are not receiving CAR‑T therapy, as well as pregnant or breastfeeding individuals, those unable to consent, or those under legal protection will not be eligible and therefore will not directly benefit from this protocol, and patients whose ICANS arises from mechanisms unrelated to extracellular vesicles may also see no direct benefit.

Why it matters

Potential benefit: If successful, this work could enable earlier detection of ICANS or point to new prevention strategies that reduce neurological harm from CAR‑T therapy.

How similar studies have performed: Preliminary human and animal data suggest extracellular vesicles change with neuroinflammation, but this specific temporal, multimodal characterization around ICANS is largely novel and unproven.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Patient aged over 18,
* Patient for whom CAR-T treatment is indicated,
* Patient affiliated to a social security system
* Patient who give his consent to participate in the study.

Exclusion Criteria:

* Pregnant or breastfeeding woman,
* Patient unable to understand informed consent,
* Patient under legal protection.

Where this trial is running

Saint-Etienne

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Immune Effector Cell Associated Neurotoxicity SyndromeCAR-T CellICANSEVs
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.