CD70-targeted CAR-T therapy for CD70-positive advanced solid tumors

Clinical Study of CD70-Targeted Chimeric Antigen Receptor T Lymphocytes (CAR-T) in Advanced CD70-Positive Malignant Solid Tumors

This trial will try CD70-targeted CAR-T cell therapy in adults with CD70-positive advanced renal, lung, ovarian, cervical, or anaplastic thyroid cancers who have exhausted standard treatments.

Quick facts

• Phase: PHASE1
• Study type: Interventional
• Enrollment: 90 (estimated)
• Ages: 18 Years and up
• Sex: All
• Sponsor: Chongqing Precision Biotech Co., Ltd (industry)
• Drugs / interventions: CAR-T, chemotherapy, prednisone
• Locations: 1 site (Hefei, Anhui)
• Trial ID: NCT07181720 on ClinicalTrials.gov

What this trial studies

This is a phase 1, single-arm, open-label study using dose-escalation followed by dose-expansion to test CD70-targeted CAR-T cell preparations in patients whose tumors show strong CD70 expression (IHC 3+). Patients are assigned to one of three parallel infusion routes — intravenous, intrapleural, or intraperitoneal — and each route is studied separately. Part A uses a 3–6 patient per dose level escalation to identify a recommended dose, and Part B expands that recommended dose to gather additional safety and preliminary efficacy data. The study will collect safety, pharmacokinetic, and early anti-tumor activity information to inform dosing and infusion schedules.

Who should consider this trial

Good fit: Adult patients (≥18 years) with histologically or cytologically confirmed advanced/metastatic solid tumors that are CD70-positive by IHC (3+), with measurable disease, ECOG 0–2, expected survival ≥12 weeks, and who have failed or are intolerant of standard second-line treatments.

Not a fit: Patients whose tumors do not express CD70, who have poor organ function, ECOG >2, severe uncontrolled psychiatric disorders, or who are unsuitable for the required infusion/monitoring procedures are unlikely to benefit from this study.

Why it matters

Potential benefit: If successful, the therapy could reduce tumor burden and extend disease control for patients with CD70-positive advanced tumors who have few remaining treatment options.

How similar studies have performed: CAR-T therapies have shown major success in blood cancers, but CD70-targeted CAR-T for solid tumors is relatively novel with limited early-phase or preclinical signals so far.

Eligibility criteria

Inclusion Criteria:

1. Age ≥18 years, regardless of gender;
2. Histologically or cytologically confirmed advanced/metastatic solid tumors (tumors with positive CD70 expression, confirmed histopathological ly with IHC 3+ score);
3. Failed or intolerant to standard second-line treatments (at least one of the following: tyrosine kinase inhibitors (TKIs), poly(ADP-ribose) polymerase inhibitors (PARPi), anti-angiogenic therapy; disease progression or inability to tolerate surgery, chemotherapy, radiotherapy, or targeted therapy);
4. At least one measurable lesion per RECIST 1.1 criteria, with measurable lesions defined as:

   1. Extranodal lesions with a long axis ≥10mm on CT scan;
   2. Lymph node lesions with a short axis ≥15mm on CT scan;
   3. CT slice thickness ≤5mm.
5. ECOG performance status of 0-2 ;
6. Expected survival ≥12 weeks;
7. No history of severe psychiatric disorders;
8. Adequate organ function as defined by the following:

   1. Hematology: White blood cell count \>2.0×10⁹/L, neutrophils \>0.8×10⁹/L, lymphocytes \>0.5×10⁹/L, platelets \>50×10⁹/L, hemoglobin \>90g/L;
   2. Cardiac: Echocardiogram showing left ventricular ejection fraction (LVEF) ≥50%, and ECG with no significant abnormalities;
   3. Renal: Serum creatinine ≤2.0×ULN;
   4. Hepatic: ALT and AST ≤3.0×ULN (may be relaxed to ≤5.0×ULN in cases with liver tumor infiltration); total bilirubin ≤2.0×ULN (may be relaxed to ≤3.0×ULN in cases with Gilbert's syndrome or liver tumor infiltration);
   5. Oxygen saturation ≥92% without supplemental oxygen;
9. Ability to undergo single or venous blood collection, with no contraindications to cellular collection;
10. Female subjects must agree to use reliable contraception (excluding fertility awareness methods) from the time of informed consent until 1 year after CAR-T cell infusion;
11. Subject or authorized guardian agrees to participate in the trial and signs the informed consent form (ICF), indicating understanding of the trial's purpose and procedures and willingness to participate.

Exclusion Criteria:

1. Prior treatment with anti-CD70 therapies;
2. Active/symptomatic central nervous system (CNS) metastasis or meningeal metastasis: Subjects with treated brain metastases are eligible if treatment was completed ≥4 weeks prior to screening and there is no evidence of progression on imaging;
3. Prior treatments within specified time frames:

   1. Participation in other interventional clinical trials within 3 months before cell infusion (for unapproved drugs, the last dose must be ≥3 months prior; for approved drugs, ≥5 half-lives prior to cell infusion);
   2. Received chemotherapy or targeted therapy within 2 weeks prior to blood collection or within 5 half-lives of the drug (whichever is shorter);
   3. Received \>10mg/day prednisone (or equivalent) within 2 weeks prior to blood collection, unless for adrenal replacement or inhaled/local steroids (except for active autoimmune disease);
   4. Received live attenuated vaccines within 4 weeks prior to screening;
4. Active infection requiring systemic treatment or uncontrolled infection within 1 week before screening;
5. History of any other malignancy within the past 3 years, except for treated and stable non-melanoma skin cancer or malignancies treated with curative intent and no evidence of active disease for ≥3 years;
6. Cardiovascular conditions:

   1. NYHA Class III or IV heart failure;
   2. Myocardial infarction or coronary artery bypass graft (CABG) within 6 months prior to screening;
   3. Clinically significant ventricular arrhythmias or unexplained syncope (excluding vasovagal or dehydration);
   4. Severe non-ischemic cardiomyopathy;
7. Active or uncontrolled autoimmune diseases such as Crohn's disease, rheumatoid arthritis, systemic lupus erythematosus, systemic vasculitis, etc.;
8. Positive for HBsAg or HBcAb with elevated HBV DNA in peripheral blood; positive for HCV antibodies with detectable HCV RNA levels; positive for HIV antibodies; positive syphilis test;
9. Toxicity from prior anti-tumor treatments has not resolved to baseline or ≤grade 1, except for alopecia or peripheral neuropathy;
10. History of venous thromboembolism (e.g., pulmonary embolism) requiring ongoing anticoagulation treatment, or meeting one of the following criteria:

    1. Severe bleeding (grade 3 or 4) lasting for ≥30 days;
    2. Post-thrombotic sequelae (e.g., persistent dyspnea and hypoxia) due to venous thromboembolism;
11. Pregnant or breastfeeding women;
12. Other conditions that, in the opinion of the investigator, make the subject unsuitable for participation in the trial.

Where this trial is running

Hefei, Anhui

• The 901 Hospital of Joint Logistics Support Force of People Liberation Army — Hefei, Anhui, China (RECRUITING)

Study contacts

• Principal investigator: Donglai Lv, MD — The 901 Hospital of Joint Logistics Support Force of People Liberation Army
• Study coordinator: Donglai Lv, MD

Conditions: Renal Cell Carcinoma, Lung Cancer, Anaplastic Thyroid Carcinomas, Ovarian Cancer, Cervical Cancer, Thymic Carcinoma