Analyzing T-Cell Activation in Lymphoma Patients with HLH

Observational Study on the Application of Flow Cytometry for the Study of T-Cell Populations in Lymphoma-associated Hemophagocytic Lymphohistiocytosis

Observational Fondazione Policlinico Universitario Agostino Gemelli IRCCS · NCT06585124

This study is trying to see if measuring T cell activity in patients newly diagnosed with aggressive lymphoma can help predict and improve the diagnosis of a serious condition called HLH.

Quick facts

Study typeObservational
Enrollment150 (estimated)
Ages18 Years and up
SexAll
SponsorFondazione Policlinico Universitario Agostino Gemelli IRCCS Academic / other
Locations1 site (Rome, Lazio)
Trial IDNCT06585124 on ClinicalTrials.gov

What this trial studies

This observational study aims to investigate the relationship between T cell activation and hemophagocytic lymphohistiocytosis (HLH) in patients newly diagnosed with aggressive lymphomas. It will involve the collection of peripheral blood samples for flow cytometric analysis to identify T-lymphocyte activation profiles and compare these with established diagnostic scores for HLH. The study seeks to predict the occurrence of lymphoma-associated HLH and identify new markers for early diagnosis, ultimately improving patient outcomes. A total of 150 patients will be enrolled within one year of their lymphoma diagnosis.

Who should consider this trial

Good fit: Ideal candidates for this study are adults over 18 years old who have been newly diagnosed with aggressive lymphomas.

Not a fit: Patients with non-aggressive lymphomas or those under 18 years old may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to earlier diagnosis and better management of HLH in lymphoma patients, potentially improving survival rates.

How similar studies have performed: Previous studies have shown promise in using T-cell activation profiling for HLH diagnosis in pediatric populations, suggesting potential applicability in adult cases.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

1. Age \> 18 years
2. Diagnosis at onset of aggressive lymphoma including the following histotypes:

   * Hodgkin lymphoma
   * Transformed B cell lymphomas;
   * Diffuse large B-cell lymphomas (diffuse large B-cell lymphoma NOS; T-cell/histiocyte-rich B-cell lymphoma; High grade B-cell lymphoma/high grade B-cell lymphoma with MYC and BCL2 rearrangement ALK-positive large B-cell lymphoma; Large B-cell lymphoma with IRF4 rearrangement; High grade B-cell lymphoma with 11q alterations; Lymphomatoid granulomatosis; EBV-positive large B-cell lymphoma Large B-cell lymphoma associated with chronic inflammation; Fibrin-associated large B-cell lymphoma; Fluid overload-associated large B-cell lymphoma; Plasmoblastic lymphoma; Immune-privileged site B-cell lymphoma Primary cutaneous leg-type large B-cell lymphoma; Intravascular large B-cell lymphoma; Primary mediastinal large B-cell lymphoma; Mediastinal grey zone lymphoma; High grade NOS B-cell lymphoma)
   * Burkitt lymphoma
   * KSHV/HHV8 a ssociatedlymphomas
   * Lymphomas associated with immunodeficiency or immune dysregulation
   * Mature T-cell-derived lymphomas (NOS peripheral T-cell lymphoma; Nodal follicular helper T-cell lymphoma; Anaplastic large cell lymphoma; Nodal and extranodal EBV-positive T/NK-cell lymphomas; Hepatosplenic T-cell lymphoma; Enteropathy-associated intestinal T-cell lymphoma, epitheliotropic monomorphic and NOS; Subcutaneous T-cell lymphoma similar to panniculitis)
3. Informed consent to the use of biologic materials for studies related to the present proposal.

Exclusion Criteria:

1. Diagnosis of indolent non-Hodgkin's lymphoma or diagnoses other than those listed in the inclusion criteria
2. Prolonged steroid therapy, defined as lasting more than 15 days or high doses of steroid, exceeding 1 mg/kg
3. Age ≤ 18 years;

Where this trial is running

Rome, Lazio

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions LymphomaHemophagocytic LymphohistiocytosesFlow CytometryT cell activationlymphomaHLH
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.