Whole-food beef protein and amino acid oxidation in young adults

Amino Acid Oxidation of Whole-Food Protein: a Dose-Response Crossover Trial in Young Adults

Not applicable Interventional Maastricht University Medical Center · NCT07547176

This test will see how three dinner doses of minced beef affect 24-hour amino acid oxidation in healthy 18–35-year-olds.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment20 (estimated)
Ages18 Years to 35 Years
SexAll
SponsorMaastricht University Medical Center Academic / other
Locations1 site (Maastricht, Limburg)
Trial IDNCT07547176 on ClinicalTrials.gov

What this trial studies

In a randomized crossover design, 20 healthy young adults (ages 18–35) consume dinners providing 0.32, 0.74, and 1.32 g protein·kg body mass−1 (equivalent to 1, 2, and 4 minced beef patties) across three separate experimental visits. Amino acid oxidation from the ingested protein is measured over a 24-hour period after each meal. Participants serve as their own controls so dose-response relationships can be compared within individuals. The study focuses on whole-food protein (minced beef) rather than isolated protein supplements.

Who should consider this trial

Good fit: Healthy males and females aged 18–35 with BMI 18.5–30 kg/m2 who are recreationally active, consume beef, and are not taking medications that affect protein metabolism are ideal candidates.

Not a fit: People who are vegan or vegetarian, smokers, pregnant women, those with diagnosed GI disorders, or those taking medications that affect protein metabolism are unlikely to benefit from participation.

Why it matters

Potential benefit: If successful, the results could help define practical whole-food protein portions that reduce amino acid waste and inform dietary protein recommendations for young adults.

How similar studies have performed: Prior dose-response studies using isolated proteins and mixed meals have shown dose-dependent amino acid oxidation, but comparable data using whole-food beef are limited.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Aged between 18-35 years
* BMI between 18.5-30 kg/m2
* Healthy, recreationally active (exercise at least once per two weeks and a maximum of four days per week)
* No physical limitations (i.e., able to perform all activities associated with daily living independently)

Exclusion Criteria:

* Smoking
* Vegan, vegetarian, or does not consume beef protein
* Diagnosed musculoskeletal disorders
* Use of any medications known to affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications)
* Chronic use of gastric acid-suppressing medication or anti-coagulants
* Unstable weight over the last three months
* Diagnosed GI tract disorders or diseases
* Blood donation in the past 2 months
* Females: pregnancy

Where this trial is running

Maastricht, Limburg

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Protein Metabolism
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.