Using PCR to improve diagnosis and management of infective endocarditis

The Role of Polymerase Chain Reaction in the Management of Patients With Infective Endocarditis: Modern Diagnostic and Therapeutic Concepts

Not applicable Interventional Fondazione Policlinico Universitario Agostino Gemelli IRCCS · NCT05791357

This study tests if a special blood test can help doctors better diagnose and manage infective endocarditis in patients who need heart surgery.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment100 (estimated)
Ages18 Years and up
SexAll
SponsorFondazione Policlinico Universitario Agostino Gemelli IRCCS Academic / other
Locations2 sites (Roma and 1 other locations)
Trial IDNCT05791357 on ClinicalTrials.gov

What this trial studies

This study aims to prospectively evaluate the added diagnostic value of broad range polymerase chain reaction (PCR) targeting the 16 ribosomal DNA in patients diagnosed with infective endocarditis who are candidates for surgical therapy. The study is structured into four phases: screening, pre-operative clinical assessment, cardiac surgery, and follow-up. During these phases, patients will undergo various assessments including blood cultures, imaging, and intraoperative data collection to enhance the understanding of their condition and treatment outcomes. Follow-up evaluations will monitor patients' clinical status post-surgery.

Who should consider this trial

Good fit: Ideal candidates for this study are adults over 18 years old diagnosed with infective endocarditis requiring heart valve replacement surgery.

Not a fit: Patients under 18 years old or those unable to provide informed consent will not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to improved diagnostic accuracy and management strategies for patients with infective endocarditis.

How similar studies have performed: While the use of PCR in infectious disease diagnostics is established, this specific application in infective endocarditis is novel and has not been extensively tested.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

1. patients older than 18 years, with diagnosis of IE on a native or prosthetic valve and with a surgical indication for heart valve replacement using extracorporeal circulation (CPB).
2. patients who have signed informed consent to the partecipation of the study

Exclusion criteria:

1. patients younger than 18 years
2. Failure to sign consent for personal data processing and/or study participation
3. Participation in other experimental studies
4. Patients who did not complete the examinations under study (culture test on blood and excised valve, molecular tests)

Where this trial is running

Roma and 1 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Endocarditis Infective
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.