Treatment of Alpha-1 Antitrypsin Deficiency with KB408

A Phase 1 Study of Inhaled KB408 for the Treatment of Alpha-1 Antitrypsin Deficiency

This study is testing a new treatment called KB408 to see if it can help adults with alpha-1 antitrypsin deficiency breathe better by delivering a key protein directly to their lungs.

Quick facts

• Phase: Phase 1
• Study type: Interventional
• Enrollment: 15 (estimated)
• Ages: 18 Years to 70 Years
• Sex: All
• Sponsor: Krystal Biotech, Inc. (Industry-sponsored)
• Locations: 3 sites (Gainesville, Florida and 2 other locations)
• Trial ID: NCT06049082 on ClinicalTrials.gov

What this trial studies

This study evaluates the safety and pharmacodynamics of KB408, a novel therapy designed to deliver functional human SERPINA1 to the airways of adults with alpha-1 antitrypsin deficiency (AATD) using a nebulization method. Participants will be grouped into cohorts based on their dosage levels, including those currently receiving intravenous AAT augmentation therapy. The study aims to assess the effects of KB408 in individuals with specific genetic profiles associated with AATD, particularly the PI*ZZ or PI*ZNull genotypes.

Who should consider this trial

Good fit: Ideal candidates are adults aged 18 to 70 with a genetically confirmed diagnosis of AATD and specific genotypes.

Not a fit: Patients with severe pulmonary function impairment or those not meeting the genetic criteria for AATD may not benefit from this study.

Why it matters

Potential benefit: If successful, this treatment could significantly improve lung function and quality of life for patients with alpha-1 antitrypsin deficiency.

How similar studies have performed: While this approach is innovative, similar gene therapy strategies have shown promise in other conditions, suggesting potential for success.

Eligibility criteria

Inclusion Criteria:

1. The subject or legally authorized representative must have read, understood, and signed an Institutional Review Board (IRB) approved Informed Consent Form and must be willing and able to comply with study procedures and instructions.
2. Subject is aged ≥18 to ≤70 years, at the time of informed consent.
3. Subject has a genetically confirmed diagnosis of AATD with a PI\*ZZ or PI\*ZNull genotype.
4. Cohort 2b and Cohort 3: Subjects receiving AAT augmentation therapy must be willing to washout for at least 10 days prior to Screening and be willing to remain off augmentation therapy for the duration of the study.
5. Cohort 2b and Cohort 3: Serum AAT level \<11 μM at Screening.
6. Willing to remain on a stable regimen of treatment during the study.
7. Resting oxygen saturation ≥92% on room air at Screening.
8. Clinically stable and in good general health, except for AATD, as determined by the Investigator.

Exclusion Criteria:

1. Pulmonary function test with percent predicted forced expired volume in 1 second (ppFEV1) after inhalation of a bronchodilator is \<40% at Screening.
2. Diffusing capacity of the lungs for carbon monoxide (DLCO) \<30 percent predicted (historical DLCO within 2 years prior to Screening without any intervening change in clinical status since the measurement was taken, or as measured at Screening).
3. Known ongoing or history of clinically significant pulmonary impairment other than AATD.
4. A pulmonary exacerbation within six weeks (42 days) of first dose.
5. Initiation of any new chronic therapy or any change in ongoing therapy routine within 28 days of first dose.
6. Participation in another interventional clinical study or treatment with an investigational agent within 30 days or 5 half-lives, whichever is longer, of first dose. Previous treatment with a genetic therapy for AATD, where the investigational product was demonstrated to be non-efficacious, is not exclusionary.
7. History of or listed for solid organ transplantation or has undergone major lung surgery (e.g., lobectomy) within 6 months of first dose.
8. Any clinical condition or illness (including a history or current evidence of substance abuse or dependence) that, in the opinion of the Investigator, would impact a subject's ability to complete all study-related procedures and/or poses an additional risk to the assessment of safety of KB408.
9. An active oral herpes infection 30 days prior to the first dose.
10. Clinically significant hepatic dysfunction defined as any one of the following:

    1. AST and ALT ≥3× upper limit of normal (ULN) at Screening
    2. Total bilirubin ≥2× ULN at Screening (unless associated with Gilbert's syndrome)
    3. Evidence of liver cirrhosis with clinical manifestations of portal hypertension (e.g., ascites, encephalopathy, variceal hemorrhage)
11. History of cigarette smoking or any other tobacco use, or use of e-cigarettes or other recreational inhalant, within 6 months of Screening.
12. Unwilling to refrain from smoking, e-cigarette use, or vaping throughout the duration of the study.
13. A positive urine cotinine result that is consistent with active smoking at Screening. (A positive cotinine test due to nicotine replacement therapy for the purpose of smoking cessation, as attested by the Investigator, is allowed.)
14. Abnormal hematology or chemistry testing at Screening as defined below, or any other clinically significant abnormalities that the Investigator believes may interfere with the assessment of safety of the study treatment.

    * Platelet count \<100×10\^9/L
    * Hemoglobin \<9 g/dL
    * White blood cell count \<3 or \>15×10\^9/L
    * Sodium \<130 or \>150 mmol/L
    * Potassium \<3 or \>5.5 mmol/L
    * Carbon dioxide \<16 mmol/L
    * Creatinine \>2 mg/dL
15. Subject is known to be noncompliant or is unlikely to comply with the requirements of the study protocol, in the opinion of the Investigator.
16. Females who are pregnant or nursing.
17. Subject who is unwilling to comply with contraception requirements per protocol

Where this trial is running

Gainesville, Florida and 2 other locations

• University of Florida, Gainesville — Gainesville, Florida, United States (Recruiting)
• Medical University of South Carolina — Charleston, South Carolina, United States (Recruiting)
• Renovatio Clinical — The Woodlands, Texas, United States (Recruiting)

Study contacts

• Study coordinator: David Sweet, MD, PhD

Conditions Alpha 1-Antitrypsin Deficiency