Study of drug interactions in healthy individuals using artemether-lumefantrine and amodiaquine

A Randomized, Open-Label Study to Evaluate Potential Pharmacokinetic Interactions of Orally Administered Artemether-lumefantrine and Amodiaquine in Healthy Adult Subjects

This study is testing how the combination of two malaria drugs, artemether-lumefantrine and amodiaquine, affects healthy adults to see if they work well together and are safe.

Quick facts

• Phase: Phase 1
• Study type: Interventional
• Enrollment: 16 (estimated)
• Ages: 18 Years to 60 Years
• Sex: All
• Sponsor: University of Oxford (Academic / other)
• Locations: 1 site (Bangkok)
• Trial ID: NCT04080895 on ClinicalTrials.gov

What this trial studies

This open-label pharmacokinetic study involves 16 healthy Thai subjects aged 18-60 years to evaluate the safety, tolerability, and pharmacological interactions of the drug combination artemether-lumefantrine and amodiaquine. Participants will undergo screening assessments to ensure they meet eligibility criteria, including being free from HIV-1 and hepatitis B and C. The study will consist of multiple visits where subjects will be randomized to receive the study drugs under controlled conditions. All laboratory assessments will be conducted in a fasting state to ensure accurate results.

Who should consider this trial

Good fit: Ideal candidates are healthy males and females aged 18-60 years who are non-smokers and meet specific health criteria.

Not a fit: Patients with existing health conditions or those who are smokers may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could enhance the understanding of drug interactions and improve treatment protocols for malaria.

How similar studies have performed: Other studies have shown success in evaluating drug interactions with similar methodologies, indicating a promising approach.

Eligibility criteria

Inclusion Criteria:

1. Healthy as judged by a responsible physician with no abnormality identified on a medical evaluation including medical history and physical examination.
2. Male or female non-smoker aged between 18 years to 60 years.
3. A female is eligible to enter and participate in this study if she is:

   * of non-childbearing potential including pre-menopausal females with documented (medical report verification) hysterectomy or double oophorectomy
   * or postmenopausal defined as 12 months of spontaneous amenorrhea or 6 months of spontaneous amenorrhea with serum follicle stimulating hormone levels \>40 mIU/mL or 6 weeks postsurgical bilateral oophorectomy with or without hysterectomy
   * or of childbearing potential, has a negative serum pregnancy test at screening and prior to start the study drug in each period, and agrees to abstain from sexual intercourse or use effective contraceptive methods (e.g., intrauterine device, hormonal contraceptive drug, tubal ligation or female barrier method with spermicide) during the study until completion of the follow-up procedures
4. A male is eligible to enter and participate in this study if he: agrees to abstain from sexual intercourse with females of childbearing potential or lactating females; or is willing to use a condom/spermicide, during the study until completion of the follow-up procedures.
5. Normal electrocardiogram (ECG) with QTc \<450 msec.
6. Willingness and ability to comply with the study protocol for the duration of the trial.

Exclusion Criteria:

1. Females who are pregnant, trying to get pregnant, or are lactating.
2. The subject has evidence of active substance abuse that may compromise safety, pharmacokinetics, or ability to adhere with protocol instructions.
3. A positive pre-study hepatitis B surface antigen, positive hepatitis C antibody, or positive human immunodeficiency virus-1 (HIV-1) antibody result at screening.
4. Subjects with a personal history of cardiac disease, symptomatic or asymptomatic arrhythmias, syncopal episodes, or additional risk factors for torsades de pointes (heart failure, hypokalemia) or with a family history of long QT syndrome, Brugada syndrome, or sudden cardiac death.
5. Abnormal serum creatinine (Scr) and estimated glomerular filtration rate (eGFR) \<70 mL/min as determined by CKD-EPI equation
6. History of alcohol or substance abuse or dependence within 6 months of the study.
7. Use of prescription or non-prescription drugs except paracetamol at doses of up to 2 grams/day, including vitamins, herbal and dietary supplements (including St. John's Wort) within 7 days (or 14 days if the drug is a potential enzyme inducer) or 5 times the drug half-life (whichever is longer) prior to the first dose of study medication until the completion of the follow-up procedure, unless in the opinion of investigator, the medication will not interfere with the study procedures or compromise subject safety; the investigator will take advice from the manufacturer representative as necessary.
8. The subject has participated in a clinical trial and has received a drug or a new chemical entity within 30 days or 5 x half-life, or twice the duration of the biological effect of any drug (whichever is longer) prior to the first dose of study medication.
9. The subject is unwilling to abstain from ingesting alcohol within 48 hours prior to the first dose of study medication until collection of the final pharmacokinetic sample during each regimen.
10. Subjects who have donated blood to the extent that participation in the study would result in more than 300 mL blood donated within a 30-day period. Note: This does not include plasma donation.
11. Subjects who have a history of allergy to the study drug or drugs of this class, or a history of drug or other allergy that, in the opinion of the investigator, contraindicates participation in the trial. In addition, if heparin is used during pharmacokinetic sampling, subjects with a history of sensitivity to heparin or heparin-induced thrombocytopenia should not be enrolled.
12. Lack of suitability for participation in this study, including but not limited to, unstable medical conditions, systemic disease manifested by tendency to granulocytopenia e.g. rheumatoid arthritis and lupus erythematosus that in the opinion of the investigator would compromise their participation in the trial.
13. AST or ALT \>1.5 times the upper limit of normal (ULN)
14. Subjects with history of renal disease, hepatic disease, and/or cholecystectomy
15. Abnormal methemoglobin level (more than 3 mg/dL).
16. History of antimalarial drugs use including but not limited to mefloquine, chloroquine, primaquine, artesunate, piperaquine and pyronaridine treatment within 6 months.
17. Subject who have received quinacrine in last 30 days.

Where this trial is running

Bangkok

• Faculty of Tropical Medicine, Mahidol University — Bangkok, Thailand (Recruiting)

Study contacts

• Study coordinator: Borimas Hanboonkunupakarn, MD

Conditions Pharmacokinetic; Healthy; Drug Combination; Artemether-lumefantrine; Amodiaquine